Effect of Extender Compositions, Glycerol Levels, and Thawing Rates on Motility and Fertility of Cryopreserved Wild African Catfish (Clarias gariepinus) Sperm

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dc.authoridBOZKURT, Yusuf/0000-0002-6162-2466
dc.contributor.authorBozkurt, Yusuf
dc.contributor.authorYavas, Ilker
dc.date.accessioned2024-09-18T19:52:28Z
dc.date.available2024-09-18T19:52:28Z
dc.date.issued2017
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractThe aim of this study was to determine the effect of different extenders, glycerol levels, and thawing rates on post-thaw sperm motility and fertilization ability of cryopreserved African catfish (Clarias gariepinus) sperm. Having determined the main spermatological properties (volume, motility, motility duration, spermatozoa concentration and pH), the pooled ejaculates were diluted with 3 different extenders containing different glycerol levels (5, 10 and 15%) respectively. Dilution ratio was 1:10 and the diluted sperm was packaged in 0.25 ml straws and left for 10 min equilibration at 4 degrees C. Following equilibration, the straws were exposed to liquid nitrogen vapor for 10 min and plunged into liquid nitrogen (-196 degrees C), and then exposed to different thawing rates (30 degrees C/20s and 40 degrees C/20s) to determine sperm motility and post-thaw motility duration. The highest post-thaw sperm motility, motility duration, and fertilization rate was 85%, 81s, and 95% respectively when sperm was frozen with the extender (ACSE 3) containing 15% glycerol (p < 0.05). The protocol reported in this study can be successfully used for cryopreservation of African catfish sperm.en_US
dc.identifier.issn0792-156X
dc.identifier.scopus2-s2.0-85013250300en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12483/7442
dc.identifier.volume69en_US
dc.identifier.wosWOS:000393911100001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherIsraeli Journal Of Aquaculture-Bamidgehen_US
dc.relation.ispartofIsraeli Journal of Aquaculture-Bamidgehen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAfrican catfishen_US
dc.subjectClarias gariepinusen_US
dc.subjectspermen_US
dc.subjectcryopreservationen_US
dc.subjectglycerolen_US
dc.subjectfertilizationen_US
dc.titleEffect of Extender Compositions, Glycerol Levels, and Thawing Rates on Motility and Fertility of Cryopreserved Wild African Catfish (Clarias gariepinus) Spermen_US
dc.typeArticleen_US

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