Homocysteine Influx and Efflux: Participation of Erythrocytes in Homocysteine Homeostasis of the Plasma

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dc.contributor.authorYildiz, Deniz
dc.contributor.authorCivi, Zuleyha
dc.contributor.authorCakir, Yeliz
dc.date.accessioned2024-09-18T20:26:46Z
dc.date.available2024-09-18T20:26:46Z
dc.date.issued2010
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractOne of the main objective of the present study was to determine if erythrocytes play a role in regulation of plasma homocysteine concentration. Another objective was to investigate if erythrocytes convert homocystine into homocysteine. In the present study, we exposed erytrocytes to different concentrations of homocysteine and then measured the nonprotein sulfhydryl (NPSH) concentrations. Erythrocytes treated in the same manner were later utilized for the homocysteine efflux studies. The effect of temperature on the influx and the efflux processes were also evaluated. We also determined the rate of homocysteine influx in the presence of different amino acids. The homocysteine influx studies demonstrated that erythrocytes can respond to increases in homocysteine concentration in the extracellular media and influx homocysteine in a concentration-dependent manner. NPSH concentrations in erythrocytes treated with 1 mM homocysteine reached to 1.47 +/- 0.01 mu mol/ml erythrocyte in 1 h whereas this concentration reached to 2.01 +/- 0.1 mu mol/ml erythrocyte in 3 mM homocysteine treated erytrocytes. The homocysteine efflux is also determined to be time-and concentration -dependent. Extracellular concentration of NPSH in 1 mM homocysteine pre-treated erythrocytes reached to 0.266 +/- 0.02 mu mol/ml erythrocyte in 1 h whereas this concentration reached to 0.64 +/- 0.01 mu mol/ml erythrocyte with 3 mM homocysteine pre-treated erythrocytes. Our results also indicate that erythrocytes convert extracellulary applied homocystine into homocysteine. Depending on our results, it could be concluded that eryhtrocytes may play a significant role in the regulation of plasma homocysteine homeostasis.en_US
dc.identifier.endpage254en_US
dc.identifier.issn2147-1762
dc.identifier.issue3en_US
dc.identifier.startpage249en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12483/10523
dc.identifier.volume23en_US
dc.identifier.wosWOS:000421108700001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherGazi Univen_US
dc.relation.ispartofGazi University Journal of Scienceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectHomocysteineen_US
dc.subjectHomocystineen_US
dc.subjectErythrocytesen_US
dc.subjectMembrane transporten_US
dc.titleHomocysteine Influx and Efflux: Participation of Erythrocytes in Homocysteine Homeostasis of the Plasmaen_US
dc.typeArticleen_US

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