Preliminary characterization of race-specific elicitors from Peronospora parasitica and their ability to elicit phenolic accumulation in Arabidopsis
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Dosyalar
Tarih
2003
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Priel Publ
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Intercellular washing fluid (IWF) obtained from the susceptible Arabidopsis accession Ws-eds1 inoculated with Peronospora parasitica isolate Emoy-2, contained an elicitor of necrosis with ecotype specificity towards Arabidopsis accessions with particular resistance genes. This elicitor caused necrosis on the highly resistant accessions La-er, Nd-1 and partly on Col-5, but not on the susceptible accessions Ws-eds1 and Oy-0. In resistant plants, injection of IWF caused hypersensitive reaction (HR)-like cell collapse which was associated with the accumulation of phenolics and lignin-like material in walls of cells undergoing cell death. The elicitor is sensitive to proteinase K and pronase enzymes, heating and autoclaving but insensitive to periodate oxidation, freezing and thawing, and is not dialyzable. Results suggest that the elicitor is a protein. Fractionation experiments using size-exclusion membranes revealed that elicitor activity has a molecular weight in excess of 100 kDa.
Açıklama
Anahtar Kelimeler
race-specific elicitor, Arabidopsis, peronospora, resistance
Kaynak
Phytoparasitica
WoS Q Değeri
Q3
Scopus Q Değeri
Q2
Cilt
31
Sayı
4
