Efflux of Glutathione and Glutathione Complexes from Human Erythrocytes in Response to Inorganic Arsenic Exposure

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dc.contributor.authorYildiz, Deniz
dc.contributor.authorCakir, Yeliz
dc.date.accessioned2024-09-18T20:02:38Z
dc.date.available2024-09-18T20:02:38Z
dc.date.issued2012
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractThe objective of the present study was to investigate if arsenic exposure results in glutathione efflux from human erythrocytes. Arsenite significantly depleted intracellular nonprotein thiol level in a time- and concentration-dependent manner. The intracellular nonprotein thiol level was decreased to 0.767 +/- 0.0017 mu mol/ml erythrocyte following exposure to 10 mM of arsenite for 4 h. Extracellular nonprotein thiol level was increased concomitantly with the intracellular decrease and reached to 0.481 +/- 0.0005 mu mol/ml erythrocyte in 4 h. In parallel with the change in extracellular nonprotein thiol levels, significant increases in extracellular glutathione levels were detected. Extracellular glutathione levels reached to 0.122 +/- 0.0013, 0.226 +/- 0.003, and 0.274 +/- 0.004 mu mol/ml erythrocyte with 1, 5, and 10 mM of arsenite, respectively. Dimercaptosuccinic acid treatment of supernatants significantly increased the glutathione levels measured in the extracellular media. Utilization of MK571 and verapamil, multidrug resistance-associated protein 1 and Pgp inhibitors, decreased the rate of glutathione efflux from erythrocytes suggesting a role for these membrane transporters in the process. The results of the present study indicate that human erythrocytes efflux glutathione in reduced free form and in conjugated form or forms that can be recovered with dimercaptosuccinic acid when exposed to arsenite.en_US
dc.identifier.doi10.1007/s12011-012-9491-9
dc.identifier.endpage459en_US
dc.identifier.issn0163-4984
dc.identifier.issn1559-0720
dc.identifier.issue1-3en_US
dc.identifier.pmid22890881en_US
dc.identifier.scopus2-s2.0-84870654333en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage451en_US
dc.identifier.urihttps://doi.org/10.1007/s12011-012-9491-9
dc.identifier.urihttps://hdl.handle.net/20.500.12483/7932
dc.identifier.volume150en_US
dc.identifier.wosWOS:000312075800063en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherHumana Press Incen_US
dc.relation.ispartofBiological Trace Element Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectArseniteen_US
dc.subjectGlutathione effluxen_US
dc.subjectErythrocytesen_US
dc.titleEfflux of Glutathione and Glutathione Complexes from Human Erythrocytes in Response to Inorganic Arsenic Exposureen_US
dc.typeArticleen_US

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