Molecular and antigenic characterization of bovine herpesvirus type 1 (BoHV-1) strains from cattle with diverse clinical cases in Turkey

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dc.authoridOzkul, Aykut/0000-0001-5008-9443
dc.authoridAlkan, Feray/0000-0003-3854-6503
dc.authoridA.Farzani, Touraj/0000-0002-1392-4048
dc.contributor.authorDagalp, Seval Bilge
dc.contributor.authorFarzani, Touraj Aligholipour
dc.contributor.authorDogan, Firat
dc.contributor.authorAlkan, Feray
dc.contributor.authorOzkul, Aykut
dc.date.accessioned2024-09-18T20:04:38Z
dc.date.available2024-09-18T20:04:38Z
dc.date.issued2020
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractThe present study reports the molecular and antigenic characterization of 13 bovine herpesvirus type 1 (BoHV-1) field viruses obtained from cattle with different clinical cases in Turkey between 1992 and 2017. We selected glycoprotein C (gC) of BoHV-1 as a target to detect and/or verify presence of the virus in suspect materials followed by virus isolation (VI) in MDBK cells. In seven out of 13 BoHV-1 positive samples, cytophatic effects (CPEs) were observed in MDBK cell cultures, although only four virus samples reached a sufficient titer to use in phylogenetic assay, restriction endonuclease analysis (REA), and virus neutralization test (VNT). According to the results of sequence analysis of the 13 BoHV-1 positive samples, nine BoHV-1 field viruses were determined as BoHV-1.1 and four as BoHV-1.2. Using REA, we demonstrated that two of our isolated viruses could be categorized as BoHV-1.1 while the other two isolates were BoHV-1.2 subtypes. Differences between the BoHV-1.1 and BoHV-1.2 isolates were also detected in the VNT results by assaying 125 suspected serum samples after testing with isolated (KY748023, KY748022, KY748020, and KY748021) and reference viruses (BoHV-1 Cooper and BoHV-5 Texas 89). These results are indicating the need to correctly identify BoHV-1 field isolates to better understand the epidemiology and pathogenesis of infection. In addition, it would be useful to identify the subtypes circulating in the specific geographical area while determining vaccination preferences.en_US
dc.identifier.doi10.1007/s11250-019-02042-6
dc.identifier.endpage564en_US
dc.identifier.issn0049-4747
dc.identifier.issn1573-7438
dc.identifier.issue2en_US
dc.identifier.pmid31482481en_US
dc.identifier.scopus2-s2.0-85072179262en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage555en_US
dc.identifier.urihttps://doi.org/10.1007/s11250-019-02042-6
dc.identifier.urihttps://hdl.handle.net/20.500.12483/8289
dc.identifier.volume52en_US
dc.identifier.wosWOS:000519374900014en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofTropical Animal Health and Productionen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBoHV-1en_US
dc.subjectPhylogenetic analysisen_US
dc.subjectMolecular and antigenic characterizationen_US
dc.subjectCattleen_US
dc.subjectTurkeyen_US
dc.titleMolecular and antigenic characterization of bovine herpesvirus type 1 (BoHV-1) strains from cattle with diverse clinical cases in Turkeyen_US
dc.typeArticleen_US

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