Comparison of N-Acetyl-L-Cysteine and L-Cysteine in Respect to Their Transmembrane Fluxes

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dc.contributor.authorYildiz, D.
dc.contributor.authorArik, M.
dc.contributor.authorCakir, Y.
dc.contributor.authorCivi, Z.
dc.date.accessioned2024-09-18T20:14:59Z
dc.date.available2024-09-18T20:14:59Z
dc.date.issued2009
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractThe objective of the present study was to compare cysteine and N-acetyl-L-cysteine in respect to their transmembrane fluxes and find out which one is a better available precursor for the cells and thus better supports the intracellular glutathione synthesis. Cysteine can directly participate in glutathione synthesis, whereas N-acetyl-L-cysteine must be first deacetylated before its incorporation to glutathione. In the present study we investigated and compared the efficiencies of cysteine and N-acetyl-L-cysteine influx and efflux through the erythrocyte membrane. Erythrocytes transported both cysteine and N-acetyl-L-cysteine in a concentration-dependent manner. However, our results demonstrated that cysteine crosses the erythrocyte membranes more efficiently as compared to N-acetyl-L-cysteine. Treatment of erythrocytes with 5 mM of cysteine or N-acetyl-L-cysteine for 1 hr raised the intracellular free sulfhydryl group (free-SH) levels to 3.37 +/- 0.006 or 2.23 +/- 0.08 mu mol/ml erythrocyte, respectively. Cysteine more effectively than N-acetyl-L-cysteine restored the intracellular free-SH level depleted beforehand. In erythrocytes previously depleted of free-SH, 5 mM cysteine raised the free-SH level to 1.45 +/- 0.075 mu mol/ml within 1 hr, whereas N-acetyl-L-cysteine at the same concentration raised this level to 0.377 +/- 0.034 mu mol/ml only. The results of our study also revealed that both cysteine and N-acetyl-L-cysteine influx and efflux processes are temperature dependent indicating that their transport requires biological activity. Our results demonstrate that cysteine is a better thiol precursor for the erythrocytes. Availability of cysteine for the cells is higher than that of N-acetyl-L-cysteine.en_US
dc.identifier.doi10.1134/S1990747809020081
dc.identifier.endpage162en_US
dc.identifier.issn1990-7478
dc.identifier.issn1990-7494
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-84876583051en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage157en_US
dc.identifier.urihttps://doi.org/10.1134/S1990747809020081
dc.identifier.urihttps://hdl.handle.net/20.500.12483/9351
dc.identifier.volume3en_US
dc.identifier.wosWOS:000219811500008en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringer Heidelbergen_US
dc.relation.ispartofBiochemistry Moscow Supplement Series A-Membrane and Cell Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectcysteineen_US
dc.subjectN-acetyl-L-cysteine (NAC)en_US
dc.subjecterythrocytesen_US
dc.subjecttransmembrane transporten_US
dc.titleComparison of N-Acetyl-L-Cysteine and L-Cysteine in Respect to Their Transmembrane Fluxesen_US
dc.typeArticleen_US

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