The presence of virulence factor genes downregulates uterine AQP3 and alters glutathione peroxidase activity and uterine histopathology in canine pyometra

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dc.authoridKaya, Ufuk/0000-0002-4805-0993
dc.authoridMUSTAK, INCI BASAK/0000-0001-9180-5768
dc.authoridALTINBAS, Yunus Furkan/0000-0002-3934-8018
dc.authoridOzkan, Huseyin/0000-0001-5753-8985
dc.authoridOzoner, Ozgur/0000-0001-7354-0655
dc.contributor.authorYazlik, Murat Onur
dc.contributor.authorMustak, Inci Basak
dc.contributor.authorOzkan, Hueseyin
dc.contributor.authorVural, Sevil Atalay
dc.contributor.authorKaya, Ufuk
dc.contributor.authorOzoner, Ozgur
dc.contributor.authorMutluer, Ipek
dc.date.accessioned2024-09-18T20:02:36Z
dc.date.available2024-09-18T20:02:36Z
dc.date.issued2024
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractPresent study was designed to evaluate the role of virulence factor genes (papG, cnf1 and hylA) in the pathogenesis of canine pyometra. Antimicrobial susceptibility test and detection of virulence genes were performed Escherichia coli (E. coli) detected in uterine swab samples. Animals were divided into two groups based on the presence (VF+, n:14) or absence (VF-, n:7) of the virulence factor genes papG, cnf1 and hylA. Blood and tissue glutathione peroxidase activity, uterine histopathologic analysis and AQP3, ESR1, PGR, OXTR gene expressions were determined in both groups. Statistical analyses were performed using Stata version 15.1. All E. coli isolates were susceptible to amikacin, whereas resistant to ampicillin, amoxicillin/clavulanic acid and lincomycin. None of the isolates were susceptible to cefotaxime. E. coli isolates had at least one virulence gene. The most prevalent gene was fimH (100%), followed by fyuA (95.8%), usp (83.3%), sfa (75%), cnf1 and hlyA (70.8%) genes. Blood GPx activity was greater in VF+ animals. On the other hand, uterine tissue GPx activity was lower in VF+ group compared to the control group. Expression levels of AQP3 were upregulated more than fivefold in VF-dogs compared to the control group. In addition, AQP3 expression levels were found approximately threefold higher in VF (-) than VF (+) group (p < .05). Varying degree of inflammation noted for all animals with pyometra, but the presence of bacteria noted only in VF+ animals. In conclusion, the presence of virulence factor genes does not play a role in the histopathological degree of inflammation, the presence of bacteria was found to vary. Serum GPx activity increased in VF+ animals. While the hormone receptor expressions were similar, AQP expression was upregulated in the absence of virulence factor genes.en_US
dc.description.sponsorshipTrkiye Bilimsel ve Teknolojik Arascedil;timath;rma Kurumu [120O543]; Scientific and Technological Research Council of Turkiye (TUBITAK)en_US
dc.description.sponsorshipThis research was supported by The Scientific and Technological Research Council of Turkiye (TUBITAK) (Project number: 120O543), Turkiye.en_US
dc.identifier.doi10.1111/rda.14615
dc.identifier.issn0936-6768
dc.identifier.issn1439-0531
dc.identifier.issue5en_US
dc.identifier.pmid38798181en_US
dc.identifier.scopus2-s2.0-85194398686en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1111/rda.14615
dc.identifier.urihttps://hdl.handle.net/20.500.12483/7896
dc.identifier.volume59en_US
dc.identifier.wosWOS:001231048200001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofReproduction in Domestic Animalsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectaquaporinen_US
dc.subjectcanineen_US
dc.subjectglutathione peroxidaseen_US
dc.subjectpyometraen_US
dc.subjectvirulence factor genesen_US
dc.titleThe presence of virulence factor genes downregulates uterine AQP3 and alters glutathione peroxidase activity and uterine histopathology in canine pyometraen_US
dc.typeArticleen_US

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