Phenotypic and genotypic characterization of antimicrobial resistance of Campylobacterisolates from poultry meat in Turkey
Yükleniyor...
Tarih
2022
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Hellenic Veterinary Medical Soc
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Phenotypic and genotypic antimicrobial resistances of Campylobacter strains from 152 chicken meatsamples were investigated. A total of 19 isolates (Campylobacter jejuni=6 and Campylobacter coli=13) were identified by multiplex PCR.The C. jejuni and C. coli isolates were tested for resistance to ciprofloxacin, erythromycin, and tetracycline using the disc diffusion method. In addition, all the strains were analyzed for resistance gene tet-O and mutations in the gyrA and 23S rRNA genes by PCR.Overall, a high frequency of resistance was detected against tetracycline (14/19, 73.6%), followed by erythromycin (7/19, 36.8%), and ciprofloxacin (6/19, 31.5%). Two of C. coli isolates (15.3%) were multidrug resistant, whereas none of the C. jejuni isolates were resistant to three antibiotics the same time.Ten of the 14 Campylobacter strains (71.4%) resistant to tetracycline carried tet-O gene. Mutations the 23S rRNA and gyrA genes were identified in 57.1% (4/7), 66.6% (4/6) of the isolates resistant to erythromycin and ciprofloxacin, respectively.
Açıklama
Anahtar Kelimeler
Campylobacter, antimicrobial resistance, gyrA, tet-O, 23S rRNA, poultry meat
Kaynak
Journal of The Hellenic Veterinary Medical Society
WoS Q Değeri
Q4
Scopus Q Değeri
Q4
Cilt
73
Sayı
4
