Antimicrobial resistance and underlying mechanisms in Staphylococcus aureus isolates

Basit Öğe Kaydı
dc.contributor.authorYilmaz, Ebru Sebnem
dc.contributor.authorAslantas, Ozkan
dc.date.accessioned2024-09-18T20:59:06Z
dc.date.available2024-09-18T20:59:06Z
dc.date.issued2017
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractObjective: To investigate the antimicrobial susceptibility of 97 clinical Staphylococcus aureus (S. aureus) strains against 14 antimicrobials and corresponding resistance mechanisms. Methods: The antimicrobial susceptibility of the isolates was determined using a disk diffusion method and antimicrobial resistance genes were screened by polymerase chain reaction. Mutations responsible for ciprofloxacin and rifampicin resistance were investigated by polymerase chain reaction and DNA sequencing. Results: All isolates were found to be susceptible to vancomycin. Various rates of resistance to penicillin (83.5%), ampicillin (77.3%), erythromycin (63.9%), tetracycline (16.5%), amoxicillin/ clavulanic acid (16.5%), ciprofloxacin (15.5%), trimethoprim/sulfamethoxazole (15.5%), oxacillin (13.4%), fusidic acid (12.4%), rifampin (6.2%), clindamycin (6.2%), gentamicin (6.2%) and mupirocin (5.2%) were determined. In addition, different combinations of resistance genes were identified among resistant isolates. Ciprofloxacin resistant isolates had mutations in codon 84 (Ser84Leu) and 106 (Gly106Asp) in the gyrA gene. Mutations in grlA were mostly related to Ser80Phe substitution. Leu466Ser mutation in the rpoB gene was detected in all rifampin resistant isolates. All methicillin resistant S. aureus isolates were SCCmec type V. Conclusions: In conclusion, it was determined that the isolates were resistant to different classes of antimicrobials at varying rates and resistance was mediated by different genetic mechanisms. Therefore, continuous monitoring of resistance in S. aureus strains is necessary to control their resistance for clinically important antimicrobials.en_US
dc.description.sponsorshipScientific Research Projects Unit of Mustafa Kemal University, Hatay, Turkey [47]en_US
dc.description.sponsorshipThis study was supported by the Scientific Research Projects Unit of Mustafa Kemal University, Hatay, Turkey (Project no: 47).en_US
dc.identifier.doi10.1016/j.apjtm.2017.10.003
dc.identifier.endpage1064en_US
dc.identifier.issn1995-7645
dc.identifier.issn2352-4146
dc.identifier.issue11en_US
dc.identifier.pmid29203102en_US
dc.identifier.scopus2-s2.0-85035094127en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1059en_US
dc.identifier.urihttps://doi.org/10.1016/j.apjtm.2017.10.003
dc.identifier.urihttps://hdl.handle.net/20.500.12483/12367
dc.identifier.volume10en_US
dc.identifier.wosWOS:000417215800007en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWolters Kluwer Medknow Publicationsen_US
dc.relation.ispartofAsian Pacific Journal of Tropical Medicineen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectStaphylococcus aureusen_US
dc.subjectAntimicrobial resistanceen_US
dc.subjectResistance mechanismsen_US
dc.subjectPoint mutationen_US
dc.titleAntimicrobial resistance and underlying mechanisms in Staphylococcus aureus isolatesen_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
Tam Metin / Full Text
Boyut:
357.61 KB
Biçim:
Adobe Portable Document Format
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu