Reduced Expression of PEDF and ALDH1A1 during Spheroid Transition of Lung Cancer Cells: An In Vitro Study

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dc.authoridOkuyan, Hamza Malik/0000-0001-7616-3330
dc.contributor.authorTerzi, M. Y.
dc.contributor.authorOkuyan, H. M.
dc.contributor.authorGulbol-Duran, G.
dc.contributor.authorUrhan-Kucuk, M.
dc.date.accessioned2024-09-18T20:29:39Z
dc.date.available2024-09-18T20:29:39Z
dc.date.issued2022
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractCancer stem cells (CSCs) can initiate tumorigenesis and metastasis and show resistance against chemotherapy owing to the expression of prominent stem cell markers. CSCs are a subpopulation of highly heterogenic cancer spheroid cells. Pigment epithelium-derived factor (PEDF) is a neurotrophic, anti-tumorigenic, and anti-metastatic protein and its gene expression levels in A549 spheroids is still unknown. We aimed to compare clonogenicity and mRNA levels of PEDF, Oct4, and ALDH1A1 between A549 and spheroid cells. Spheroid and colony formation assays were performed with spheroid and A549 cells. We performed quantitative reverse transcription-polymerase chain reaction (qRT-PCR) for gene expression analysis. The clonogenic ratios for A549 and spheroids were similar to 60% and similar to 1% respectively. During spheroid formation, Oct4 mRNA level did not change but PEDF and ALDH1A1 levels decreased significantly. CSCs are characterized by elevated stem cell markers but spheroid cells consist of heterogeneous population including CSCs. In spheroid population, no increase in stem cell markers was observed. The reduced PEDF levels during spheroid transition can be a suppression mechanism of spheroid cells.en_US
dc.description.sponsorshipScientific Research Project Funds of Hatay Mustafa Kemal University [18.M. 102]en_US
dc.description.sponsorshipThis research was funded by the Scientific Research Project Funds of Hatay Mustafa Kemal University (Project no. 18.M. 102).en_US
dc.identifier.doi10.3103/S0095452722020104
dc.identifier.endpage178en_US
dc.identifier.issn0095-4527
dc.identifier.issn1934-9440
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-85128486763en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage172en_US
dc.identifier.urihttps://doi.org/10.3103/S0095452722020104
dc.identifier.urihttps://hdl.handle.net/20.500.12483/10993
dc.identifier.volume56en_US
dc.identifier.wosWOS:000784966300007en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherPleiades Publishing Incen_US
dc.relation.ispartofCytology and Geneticsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectlung canceren_US
dc.subjectPEDFen_US
dc.subjectA549en_US
dc.subjectspheroid cellen_US
dc.subjectOct4en_US
dc.subjectALDH1A1en_US
dc.titleReduced Expression of PEDF and ALDH1A1 during Spheroid Transition of Lung Cancer Cells: An In Vitro Studyen_US
dc.typeArticleen_US

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