Detection of Salmonella spp., Salmonella Enteritidis, Salmonella Typhi and Salmonella Typhimurium in cream cakes by polymerase chain reaction (PCR)

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dc.contributor.authorCan, Hayriye Yesim
dc.contributor.authorElmali, Mehmet
dc.contributor.authorKaragoz, Alper
dc.contributor.authorOner, Suleyman
dc.date.accessioned2024-09-18T20:56:50Z
dc.date.available2024-09-18T20:56:50Z
dc.date.issued2014
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractThe aims of this study were: (i) to determine the incidence of Salmonella spp. by conventional culture methods in cream cakes, (ii) to detect invA gene by PCR for the confirmation of the isolates, (iii) to analyze isolates for S. Enteritidis, S. Typhi, and S. Typhimurium by PCR based on Sdf I, ViaB, Spy gene sequences, respectively, and (iv) to identify isolates in comparison to the reference strain by DNA sequence analysis. A total of 81 unpackaged cream cake samples were obtained from different patisseries in Hatay, Turkey. Salmonella spp. was detected in 13 (16%) out of 81 cream cake samples by the conventional culture method. A total of 45 isolates from the 13 positive samples were confirmed as Salmonella spp. by PCR. Homology among the reference strain and isolates and homology within the isolates was found to be 98.97-100%. Cream cake samples analyzed in this study were found to be contaminated with Salmonella, thus posing a potential health hazard for the consumer. To protect public health, food safety management systems such as HACCP, GMP and GHP could be carried out in cream cake production.en_US
dc.identifier.endpage692en_US
dc.identifier.issn0025-8628
dc.identifier.issue11en_US
dc.identifier.scopus2-s2.0-84916882333en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage689en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12483/12104
dc.identifier.volume70en_US
dc.identifier.wosWOS:000344134800008en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherPolish Soc Veterinary Sciences Editorial Officeen_US
dc.relation.ispartofMedycyna Weterynaryjna-Veterinary Medicine-Science and Practiceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectSalmonellaen_US
dc.subjectcream cakeen_US
dc.subjectinvA geneen_US
dc.subjectsequence analysisen_US
dc.titleDetection of Salmonella spp., Salmonella Enteritidis, Salmonella Typhi and Salmonella Typhimurium in cream cakes by polymerase chain reaction (PCR)en_US
dc.typeArticleen_US

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