Genotyping, drug resistance and virulence factors of Candida species isolated from patients using long-term inhaled steroids

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dc.authoriddikmen, nursel/0000-0002-5923-400X
dc.authoridDuran, Nizami/0000-0002-2766-3491
dc.authoridCimen Acikgul, Funda/0000-0002-8904-1444
dc.contributor.authorDikmen, Nursel
dc.contributor.authorDuran, Nizami
dc.contributor.authorAy, Emrah
dc.contributor.authorCimen, Funda
dc.contributor.authorTek, Erhan
dc.date.accessioned2024-09-18T21:05:14Z
dc.date.available2024-09-18T21:05:14Z
dc.date.issued2021
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractAims In this study, it was aimed to determine the isolation frequency and species distribution of Candida species isolated from asthmatic patients using long-term inhaled steroids. It was also aimed to determine the drug resistance patterns and the frequency of erg11, HWP1, ALS1, INT1, SAP1 PLB1 genes in isolates. Methods Genotyping of Candida strains isolated from patients and healthy control group was performed by PCR-RFLP method. Drug resistance was investigated phenotypically, and the presence of erg11 resistance genes and HWP1, ALS1, INT1, SAP1 PLB1 virulence genes were investigated by PCR method. Results C albicans was the most isolated species in steroid-using patients and healthy control groups (patients: 44.2%; control group: 30.8%). C tropicalis and C glabrata were found to have the highest rates of non-albicans Candida in patients with 17.4% and 13.77%, respectively. Azole resistance was found to be significantly higher in isolates isolated from patients compared to the control group. Similarly, the presence of erg11 resistance gene was highest in C albicans (17.65%), C glabrata (12.5%) and C tropicalis (8.3%) strains in the control group, while C parapsilosis was highest in patients. (57.1%) and C glabrata (54.2%) strains. Compared to the control group, the virulence of Candida strains isolated from the patients was found to be higher. Presence of HWP1, ALS1, INT1, SAP1 and PLB1 genes in patients were determined as 72.1%, 63.9%, 68.9%, 57.38% and 54.5%, respectively. These rates were 29.4%, 35.3%, 25.5%, 17.7% and 23.5% in the healthy control group, respectively. Conclusions In asthma patients using long-term inhaled steroids, both Isolation rates of Candida species, drug resistance rates, presence of virulence genes were found to be significantly higher in patients than in the control group. We think that this may be due to the suppression of cellular immunity by long-term steroid use.en_US
dc.description.sponsorshipScientific Research Fund of Hatay Mustafa Kemal University [20, 056]; National Institutes of Health Research (NIHR) [056] Funding Source: National Institutes of Health Research (NIHR)en_US
dc.description.sponsorshipThis study was supported by the Scientific Research Fund of Hatay Mustafa Kemal University as project number 20. M.056.en_US
dc.identifier.doi10.1111/ijcp.14820
dc.identifier.issn1368-5031
dc.identifier.issn1742-1241
dc.identifier.issue11en_US
dc.identifier.pmid34487398en_US
dc.identifier.scopus2-s2.0-85115155744en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1111/ijcp.14820
dc.identifier.urihttps://hdl.handle.net/20.500.12483/13464
dc.identifier.volume75en_US
dc.identifier.wosWOS:000697320600001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofInternational Journal of Clinical Practiceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAlbicansen_US
dc.subjectGeneen_US
dc.titleGenotyping, drug resistance and virulence factors of Candida species isolated from patients using long-term inhaled steroidsen_US
dc.typeArticleen_US

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