Emergence and Characterization of Tigecycline Resistance Gene tet(X4) in ST609 Escherichia coli Isolates from Wastewater in Turkey

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dc.authoridMohsin, Mashkoor/0000-0002-5460-5780
dc.authoridLu, Xiaoyu/0000-0002-1330-5925
dc.authoridRuichao, Li/0000-0001-8333-9891
dc.authoridDisli, Huseyin Burak/0000-0001-8255-3802
dc.contributor.authorKürekci, Cemil
dc.contributor.authorLu, Xiaoyu
dc.contributor.authorCelil, Busra Gulay
dc.contributor.authorDisli, Huseyin Burak
dc.contributor.authorMohsin, Mashkoor
dc.contributor.authorWang, Zhiqiang
dc.contributor.authorLi, Ruichao
dc.date.accessioned2024-09-18T20:19:51Z
dc.date.available2024-09-18T20:19:51Z
dc.date.issued2022
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractEmergence of pathogens harboring tigecycline resistance genes incurs great concerns. Wastewater is recognized as the important reservoir of antimicrobial resistance genes. Here we characterized the phenotypes and genotypes of bacteria carrying tet(X4) from wastewater in Turkey for the first time. Four tet(X4)-positive Escherichia coli isolates were identified and characterized by PCR, Sanger sequencing, antimicrobial susceptibility testing, conjugation assays, Illumina sequencing, nanopore sequencing and bioinformatic analysis. Four tet(X4)-harboring isolates were multidrug-resistant (MDR) bacteria and the tet(X4) gene was nontransferable in four isolates. Genetic analysis revealed that tet(X4) genes in four isolates were located on plasmids co-harboring two replicons IncFIA(HI1) and IncFIB(K). However, none of the four plasmids carried genes associated with horizontal transfer of plasmids. The coexistence of bla(SHV-12)-bearing IncX3-type plasmid and tet(X4)harboring plasmid was also found in one isolate. These findings indicate that continuous surveillance of the tet(X4)-bearing isolates in different environments worldwide should be strengthened. IMPORTANCE The emergence of tigecycline resistance genes in humans and animals in China seriously threatens the clinical utility of tigecycline, but the molecular epidemiology of tigecycline-resistant bacteria in other countries remained largely unknown. Therefore, it is necessary to learn the prevalence and molecular characteristics of bacteria carrying tigecycline resistance genes, particularly the mobilizable tet(X4), in other countries. In the study, we first described the presence and molecular characteristics of the tet(X4)-positive E. coli isolates from wastewater in Turkey. Four tet(X4)-bearing isolates belonged to ST609, an E. coli clone commonly found from humans, animals and the environment. These findings highlight the importance of monitoring the tet(X4) gene in different settings globally.en_US
dc.description.sponsorshipNational Natural Science Foundation of China [32161133005]; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)en_US
dc.description.sponsorshipThis work was supported by the National Natural Science Foundation of China (no. 32161133005) and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).en_US
dc.identifier.doi10.1128/spectrum.00732-22
dc.identifier.issn2165-0497
dc.identifier.issue4en_US
dc.identifier.pmid35863037en_US
dc.identifier.scopus2-s2.0-85137138476en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1128/spectrum.00732-22
dc.identifier.urihttps://hdl.handle.net/20.500.12483/9907
dc.identifier.volume10en_US
dc.identifier.wosWOS:000825315500003en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAmer Soc Microbiologyen_US
dc.relation.ispartofMicrobiology Spectrumen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectEscherichia colien_US
dc.subjecttet(X4)en_US
dc.subjectwastewateren_US
dc.subjectST609en_US
dc.subjectbla(SHV-12)en_US
dc.titleEmergence and Characterization of Tigecycline Resistance Gene tet(X4) in ST609 Escherichia coli Isolates from Wastewater in Turkeyen_US
dc.typeArticleen_US

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