Incidence and genetic diversity of grapevine leafroll-associated virus 3 (GLRaV-3) isolates in Turkey

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dc.authoridTUNC, BAHAR/0000-0001-7990-7622
dc.contributor.authorGazel, Mona
dc.contributor.authorTunc, Bahar
dc.contributor.authorElci, Eminur
dc.contributor.authorCaglayan, Kadriye
dc.date.accessioned2024-09-18T20:25:30Z
dc.date.available2024-09-18T20:25:30Z
dc.date.issued2022
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractGrapevine leafroll-associated virus 3 (GLRaV-3) is the most important virus species within the grapevine leafroll complex. It causes significant losses to growers and vineries because of its effect on grape and wine quality. A survey was conducted in the major grape-growing provinces of Turkey in 2018 to investigate distribution and the genetic diversity of GLRaV-3 in local and foreign grapevine cultivars. The genetic diversity of GLRaV-3 isolates based on partial heat-shock protein 70 homologue (Hsp70 h), partial coat protein (CP) and partial RNA-dependent RNA polymerase (RdRp) genes were determined by RT-PCR and sequencing. The infection rate of GLRaV-3 in 141 tested grapevine samples was 13.47% by DAS-ELISA and 28.36% by RT-PCR analysis. Totally, 17 amplicons from Hsp70 h, 22 isolates from RdRp and 16 isolates from CP genes were sequenced in both directions. The sequence analysis of three genes revealed that the Turkish GLRaV-3 isolates shared 91-100% nucleotide identities with the sequences of GLRaV-3 isolated deposited in the GenBank from other parts of the world without any correlation between the distribution and geographical origin.en_US
dc.description.sponsorshipMKU-BAP project [18.M.099]en_US
dc.description.sponsorshipThis work was supported by MKU-BAP 18.M.099 project.en_US
dc.identifier.doi10.1016/j.pmpp.2022.101896
dc.identifier.issn0885-5765
dc.identifier.scopus2-s2.0-85137384730en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1016/j.pmpp.2022.101896
dc.identifier.urihttps://hdl.handle.net/20.500.12483/10332
dc.identifier.volume122en_US
dc.identifier.wosWOS:000906574800001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherAcademic Press Ltd- Elsevier Science Ltden_US
dc.relation.ispartofPhysiological and Molecular Plant Pathologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGrapevineen_US
dc.subjectGLRaV-3en_US
dc.subjectRT-PCRen_US
dc.subjectGenetic variabilityen_US
dc.titleIncidence and genetic diversity of grapevine leafroll-associated virus 3 (GLRaV-3) isolates in Turkeyen_US
dc.typeArticleen_US

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