Molecular genotyping of Echinococcus granulosus sensu stricto from human Echinococcal cysts in Hatay, Turkiye

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dc.authoridkaraarslan, kerem/0000-0003-0973-6893
dc.authoridCetinkaya, Ulfet/0000-0001-5527-3741
dc.authoridAcikgoz, Gunes/0000-0001-9118-3153
dc.contributor.authorHamamci, Berna
dc.contributor.authorAcikgoz, Gunes
dc.contributor.authorCetinkaya, Uelfet
dc.contributor.authorKilic, Erol
dc.contributor.authorKocal, Sedat
dc.contributor.authorKaraaslan, Kerem
dc.contributor.authorYetim, Tuelin Durgun
dc.date.accessioned2024-09-18T20:16:38Z
dc.date.available2024-09-18T20:16:38Z
dc.date.issued2023
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractCystic echinococcosis (CE) is one of the zoonotic infections in human, an important global health problem. It was aimed to determine the molecular characterization and phylogenetic analysis of isolates obtained from patients diagnosed with CE in Hatay province, according to the cox1 gene region. A total of 31 patients, 14 males and 17 females, with a mean age of 35.19 (+/- 14.28) years were included in the study. 35 cyst materials obtained from patients were studied. DNA isolation was performed from the samples with protoscoleces determined in the cyst fluid. One-way DNA sequencing was performed with the Sanger Sequencing Protocol through the obtained PCR products. In the study, 35 hydatid cysts of human origin were examined and protoscoleces was detected in 11 (31.43%) of them. Twenty of the patients had liver involvement, seven had lung involvement, and four had both liver and lung involvement. All the samples with protoscoleces detected were observed of PCR product with a size of approximately 446 bp. When the sequence results of the isolates were evaluated within themselves, it was seen that there were three different sequences with 99% similarity to each other. As a result, of the phylogenetic analysis, it was determined that the isolates were identified in the Echinococcus granulosus sensu stricto (E. granulosus s. s.) (G1-G3) complex. This study is thought to contribute to the epidemiology, parasite control, effective diagnosis and treatment techniques, eradication, vaccine and drug development studies of E. granulosus s. s in Turkiye.en_US
dc.identifier.doi10.1016/j.exppara.2022.108454
dc.identifier.issn0014-4894
dc.identifier.issn1090-2449
dc.identifier.pmid36587835en_US
dc.identifier.urihttps://doi.org/10.1016/j.exppara.2022.108454
dc.identifier.urihttps://hdl.handle.net/20.500.12483/9648
dc.identifier.volume245en_US
dc.identifier.wosWOS:000921195200001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAcademic Press Inc Elsevier Scienceen_US
dc.relation.ispartofExperimental Parasitologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectEchinococcus granulosusen_US
dc.subjectHydatid cysten_US
dc.subjectPCRen_US
dc.subjectMolecular epidemiologyen_US
dc.subjectHumanen_US
dc.titleMolecular genotyping of Echinococcus granulosus sensu stricto from human Echinococcal cysts in Hatay, Turkiyeen_US
dc.typeArticleen_US

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