Somatic embryogenesis, callus production, and plantlet growth in sainfoin (Onobrychis viciifolia Scop.)
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Tarih
2006
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Rsnz Publishing
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Plant parts (internode segments, young leaves, apical-axillary meristems, petioles and immature inflorescences) isolated from the fresh, full-leaved and juicy sainfoin (Onobrychis viciifolia Scop.) were used as explants in this study. The explants were cultured on B5 medium with 5.4 mu M or 10.7 mu M NAA combined with 0, 0.9, 1.4, or 2.3 mu M of kinetin and 0, 0.9, 1.3, or 2.2 mu M of BAP to determine the effects of explant types and medium compositions on the callus induction and plant regeneration. Somatic embryogenesis and organogenesis, as well as direct shoot regeneration from the explants, were observed depending on the explant types and medium composition. The effect of the phytohormone type and concentration on the callus induction rate was under explant dependency. The highest frequency of shoot regeneration was achieved from apical-axillary meristems on B5 medium with 10.7 mu MNAA and 2.3 mu M kinetin.
Açıklama
Anahtar Kelimeler
sainfoin, phytohormones, explant, somatic embryogenesis
Kaynak
New Zealand Journal of Agricultural Research
WoS Q Değeri
Q2
Scopus Q Değeri
Q1
Cilt
49
Sayı
4
