In vitro and in vivo antimicrobial effects of mastic chewing gum against Streptococcus mutans and mutans streptococci

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dc.contributor.authorAksoy, Alev
dc.contributor.authorDuran, Nizami
dc.contributor.authorKoksal, Fatih
dc.date.accessioned2024-09-18T20:54:17Z
dc.date.available2024-09-18T20:54:17Z
dc.date.issued2006
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractBackground and objective: Dental caries is associated with oral pathogens and Streptococcus mutans (S. mutans) is one of the primary cariogenic organisms. Mastic gum, from Pistacia lentiscus, has been shown to have antibacterial properties. The objective of this study was to determine antibacterial activity of mastic chewing gum against S. mutans and mutans streptococci in vitro and in vivo conditions. Setting: Cukurova University, Dental School, in 2002. Materials and methods: Antimicrobial activity of mastic gum was evaluated using standard S. mutans strain by disc diffusion method in vitro. Cytotoxicity effect of mastic gum on HEp-2 cells was evaluated by conventional haemocytometer using the trypan blue exclusion method. Clinical studies were then performed on 25 periodontally healthy volunteers. The inhibitory effect of chewing mastic gum against mutans streptococci in sativa was compared to a placebo gum. Saliva samples were taken from the subjects immediately before and after chewing the mastic gum and the placebo gum for 15 min. Additional sativa samples were collected every 30 min. The samples were inoculated onto mitis sativarius-bacitracin agar and incubated for 48 h anaerobicatty at 37 degrees C. The total number of viable bacteria was then counted. Results: Among tested solvents (chloroform, acetone petrolium ether and ethanol), it was found that the acetone was found to be more convenient than the others to dissolve the mastic gum. In the cytotoxicity assay, concentrations up to 75 mg/ml of the mastic gum were not toxic for the replication of HEp-2 cells. Thus, tower concentrations of mastic gum (20 and 50 mg/ml) were used for the experiments. In vitro experiments, the diameters of growth inhibition zones of mastic gum were in the range 9.0-27.0 mm. In the clinical trials, the mean number of bacteria in samples taken after chewing the mastic gum and placebo gum were following; at minute 15 was 112 x 10(4) +/- 268 x 10(3) and 175 x 10(4) +/- 417 x 10(3) cfu/ml, for minute 45 was 85 x 10(4) +/- 219 x 10(3) and 165 x 10(4) +/- 329 x 10(3) cfu/ml, at minute 75 was 65 x 104 100 X 103 and 160 x 104 216 x 103 cfu/ml, at minute 105 was 60 x 10(4) +/- 127 x 10(3) and 150 x 10(4) +/- 138 x 10(3) cfu/ml, and at minute 135 was 55 x 10(4) +/- 65 x 10(3) and 145 x 10(4) +/- 354 x 10(3) cfu/ml, respectively. Significantly fewer bacteria was found in saliva samples collected after chewing mastic gum compared to those after chewing paraffin (p < 0.001). Conclusions: This preliminary study showed that mastic gum had significant antibacterial activity against S. mutans and mutans streptococci and it may be a useful adjunct in the prevention of caries. (c) 2005 Elsevier Ltd. All rights reserved.en_US
dc.identifier.doi10.1016/j.archoralbio.2005.11.003
dc.identifier.endpage481en_US
dc.identifier.issn0003-9969
dc.identifier.issn1879-1506
dc.identifier.issue6en_US
dc.identifier.pmid16343417en_US
dc.identifier.scopus2-s2.0-33646382379en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage476en_US
dc.identifier.urihttps://doi.org/10.1016/j.archoralbio.2005.11.003
dc.identifier.urihttps://hdl.handle.net/20.500.12483/11719
dc.identifier.volume51en_US
dc.identifier.wosWOS:000238160400006en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherPergamon-Elsevier Science Ltden_US
dc.relation.ispartofArchives of Oral Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPistacia lentiscusen_US
dc.subjectStreptococcus mutansen_US
dc.subjectmutans streptococcien_US
dc.subjectmastic gumen_US
dc.subjectsalivaen_US
dc.subjectantibacterial activityen_US
dc.titleIn vitro and in vivo antimicrobial effects of mastic chewing gum against Streptococcus mutans and mutans streptococcien_US
dc.typeArticleen_US

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