The anticancer activity of visnagin, isolated from Ammi visnaga L., against the human malignant melanoma cell lines, HT 144

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dc.authoridAYDOGMUS OZTURK, FATMA/0000-0001-6070-7452
dc.authoridaydogmus, fatma/0000-0003-1434-2143
dc.authoridJahan, Dr. Humera/0000-0003-2052-9190
dc.contributor.authorAydogmus-Ozturk, Fatma
dc.contributor.authorJahan, Humera
dc.contributor.authorBeyazit, Neslihan
dc.contributor.authorGunaydin, Keriman
dc.contributor.authorChoudhary, Muhammad Iqbal
dc.date.accessioned2024-09-18T21:00:35Z
dc.date.available2024-09-18T21:00:35Z
dc.date.issued2019
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractMelanoma is a cancer of melanocyte cells and has the highest global incidence. There is a need to develop new drugs for the treatment of this deadly cancer, which is resistant to currently used treatment modalities. We investigated the anticancer activity of visnagin, a natural furanochromone derivative, isolated from Ammi visnaga L., against malignant melanoma (HT 144)cell lines. The singlet oxygen production capacity of visnagin was determined by the RNO bleaching method while cytotoxic activity by theMTT assay. Further, HT 144 cells treated with visnagin were alsoexposed to visible light (400 nm) for 25min to examine the illuminationcytotoxicactivity.The apoptosis was measured by flow cytometry with annexin V/PI dual staining technique. The effect of TNF-secretion on apoptosis was also investigated. In standard MTT assay, visnagin (100 mu g/mL) exhibited 80.93% inhibitory activity against HT 144 cancer celllines, while in illuminated MTT assayat same concentration it showed lesser inhibitory activity (63.19%). Visnagin was induced apoptosis due to the intracellular generation of reactive oxygen species (ROS) andshowed an apoptotic effect against HT 144 cell lines by 25.88%. However, it has no effect on TNF- secretion. Our study indicates that visnagin can inhibit the proliferation of malignant melanoma, apparently by inducing the intracellular oxidative stress.en_US
dc.description.sponsorshipResearch Fund of Istanbul University [TP-19969]en_US
dc.description.sponsorshipThis study is a part of F.A.o's Ph.D. thesis and was supported by the Research Fund of Istanbul University (Project Number: TP-19969). Prof. Dr. KerimanGunaydn would like to thank all staff of the Dr. Panjwani Center for Molecular Medicine and Drug Research (ICCBS), University of Karachi, Pakistan, for providing research facilities for her studies.en_US
dc.identifier.doi10.1007/s11033-019-04620-1
dc.identifier.endpage1714en_US
dc.identifier.issn0301-4851
dc.identifier.issn1573-4978
dc.identifier.issue2en_US
dc.identifier.pmid30694454en_US
dc.identifier.scopus2-s2.0-85061009434en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1709en_US
dc.identifier.urihttps://doi.org/10.1007/s11033-019-04620-1
dc.identifier.urihttps://hdl.handle.net/20.500.12483/12773
dc.identifier.volume46en_US
dc.identifier.wosWOS:000463996700019en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofMolecular Biology Reportsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectVisnaginen_US
dc.subjectAmmi visnaga Len_US
dc.subjectMalignant melanomaen_US
dc.subjectApoptosisen_US
dc.titleThe anticancer activity of visnagin, isolated from Ammi visnaga L., against the human malignant melanoma cell lines, HT 144en_US
dc.typeArticleen_US

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