Crystal structure and DNA binding properties of khellin oxime

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dc.authoridKaya, Kerem/0000-0002-5736-488X
dc.authoridSenel, Pelin/0000-0003-4495-8723
dc.authoridDaut Ozdemir, Ayse/0000-0001-8547-523X
dc.authoridBeyazit, Neslihan/0000-0002-5722-2400
dc.contributor.authorBeyazit, Neslihan
dc.contributor.authorKaya, Kerem
dc.contributor.authorSenel, Pelin
dc.contributor.authorOzdemir, Ayse Daut
dc.contributor.authorGolcu, Aysegul
dc.date.accessioned2024-09-18T19:54:16Z
dc.date.available2024-09-18T19:54:16Z
dc.date.issued2019
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractThe dried fruits of Ammi visnaga were subjected to Soxhlet extraction with water, followed by hexane and purified by column chromatography to yield Khellin (1). Oximation of 1 yielded khellin oxime (2), crystallized by slow evaporation from CH2Cl2:EtOAc (6:3), characterized by single-crystal X-ray diffraction method for the first time. The DNA binding affinities of the 1 and 2 have been appraised by examining their ability to bind to dsFSDNA with ultraviolet-visible and fluorescence spectroscopies, thermal denaturation and square wave voltammetry techniques. The binding constants of the both compounds with dsFSDNA are calculated by UV-vis measurements. The square wave voltammograms proved that both compounds bind to dsFSDNA by intercalative binding mode. Competitive studies with ethidium bromide (EB) have shown that 1 and 2 exhibit the ability to displace the DNA-bound EB indicating 1 and 2 bind to DNA with intercalation with EB for the intercalative binding site as a challenging displacement. (C) 2019 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipMustafa Kemal University Scientific Research Projects (HMKU BAP) [9560]en_US
dc.description.sponsorshipThe authors wish to thank Dr. Bunyamin Karagoz (for fluorescence spectrometer) and Istanbul Technical University for all experimental conditions. N. Beyazit wishes to thank Mustafa Kemal University Scientific Research Projects (HMKU BAP) through for the financial support via project number: 9560.en_US
dc.identifier.doi10.1016/j.molstruc.2019.07.019
dc.identifier.endpage457en_US
dc.identifier.issn0022-2860
dc.identifier.issn1872-8014
dc.identifier.scopus2-s2.0-85069645046en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage450en_US
dc.identifier.urihttps://doi.org/10.1016/j.molstruc.2019.07.019
dc.identifier.urihttps://hdl.handle.net/20.500.12483/7618
dc.identifier.volume1197en_US
dc.identifier.wosWOS:000487932000048en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofJournal of Molecular Structureen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAmmi visnagaen_US
dc.subjectKhellinen_US
dc.subjectKhellin oximeen_US
dc.subjectCrystal structureen_US
dc.subjectDNA bindingen_US
dc.titleCrystal structure and DNA binding properties of khellin oximeen_US
dc.typeArticleen_US

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