Kinetic properties and storage stability of catalase immobilized on to florisil

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dc.authoridTukel, Seyhan/0000-0003-2503-2781
dc.authoridAlptekin, Ozlem/0000-0002-0458-7609
dc.contributor.authorOzyilmaz, Gul
dc.contributor.authorTukel, S. Seyhan
dc.contributor.authorAlptekin, Ozlem
dc.date.accessioned2024-09-18T20:08:05Z
dc.date.available2024-09-18T20:08:05Z
dc.date.issued2007
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractThe covalent immobilization of bovine liver catalase (CAT) oil to florisil via glutaraldehyde was investigated. Optimum immobilization pH and temperature were determined as pH 6.0, 10 degrees C respectively, while the amount of initial CAT per, of carrier and immobilization time was determined as 5 mg . g(-1) and 120 min, respectively. The V-max values for free and immobilized CAT were found to be 1.7 x 10(5) and 2.0 x 10(4) mu mol H2O2 . min(-1) mg protein(-1), respectively, whereas K-M values were 33.3 mM and 1722.0 mM respectively. Operational stability was determined by using a stirred batch-type column reactor. Immobilized CAT retained about 40% of its initial activity after 50 uses. It showed higher storage stability than free CAT at 4 degrees C and 25 degrees C. Its storage stability increased with increasing relative humidity (RH) from 0 to 20% of the medium. The highest storage stability was obtained in 20% RH, however, further increase in RH from 40 to 100% significantly decreased the storage stability.en_US
dc.identifier.endpage43en_US
dc.identifier.issn0301-1208
dc.identifier.issn0975-0959
dc.identifier.issue1en_US
dc.identifier.pmid17385339en_US
dc.identifier.scopus2-s2.0-34247149283en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage38en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12483/8615
dc.identifier.volume44en_US
dc.identifier.wosWOS:000244846200006en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherNatl Inst Science Communication-Niscairen_US
dc.relation.ispartofIndian Journal of Biochemistry & Biophysicsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectcatalaseen_US
dc.subjectflorisilen_US
dc.subjectcovalent immobilizationen_US
dc.subjectstorage stabilityen_US
dc.subjectrelative humidityen_US
dc.titleKinetic properties and storage stability of catalase immobilized on to florisilen_US
dc.typeArticleen_US

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