Effect of different cryoprotectants (Glycerol, methanol and dimethyl sulfoxide) on post-thaw quality, viability, fertilization ability and dna damage of cryopreserved nile tilapia (oreochromis niloticus) spermatozoa

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dc.authorscopusid6602647463
dc.authorscopusid8840879600
dc.authorscopusid8840879500
dc.authorscopusid6508230981
dc.contributor.authorBozkurt, Yusuf
dc.contributor.authorYavaş, İlker
dc.contributor.authorBucak, Mustafa Numan
dc.contributor.authorYeni, Deniz
dc.date.accessioned2024-09-19T15:46:58Z
dc.date.available2024-09-19T15:46:58Z
dc.date.issued2019
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractBACKGROUND: Cryopreservation of sperm from different fish species requires different protocols. Therefore, it is necessary to perform studies to establish reliable procedures for each species. OBJECTIVE: Experiments were designed to analyse the effect of different types of cryoprotectants on post-thaw motility, viability and fertility as well as cryoresistance of cryopreserved Nile tilapia (Oreochromis niloticus) sperm. MATERIALS AND METHODS: Sperm samples were diluted with an ionic extender containing glycerol (Gly), methanol (MeOH) and dimethyl sulfoxide (DMSO) at ratios of 5, 10 and 15 % respectively. Diluted samples were aspirated into 0.25 ml French straws and frozen 3 cm above the surface of liquid nitrogen (LN) in a styrofoam box and stored in a LN tank. DNA damage was evaluated with the comet assay technique following cryopreservation. RESULTS: Supplementation of extender with 10% glycerol gave the highest motility rate compared with the other cryoprotectant groups (P<0.05). Differences in terms of post-thaw motility duration, cell viability and fertilization rates were not significant among treatments (P>0.05). Although Gly gave the best score (5.0 ± 0.1%, P>0.05) at the concentration of 10%, 5% Me2SO caused significant DNA damage (15.0 ± 1.0%, P<0.05) with the comet test. CONCLUSION: Gly or MeOH are more suitable cryoprotectants than DMSO for the cryopreservation of Nile tilapia sperm. © CryoLetters.en_US
dc.description.sponsorshipMustafa Kemal University Scientific Research Fund, (MKU BAP-384)en_US
dc.identifier.endpage17en_US
dc.identifier.issn0143-2044
dc.identifier.issue1en_US
dc.identifier.pmid30955026en_US
dc.identifier.scopus2-s2.0-85064503094en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage11en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12483/14876
dc.identifier.volume40en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherCryo-Lettersen_US
dc.relation.ispartofCryo-Lettersen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCryodamageen_US
dc.subjectCryoprotectanten_US
dc.subjectDNA damageen_US
dc.subjectFish sperm motilityen_US
dc.subjectNile tilapiaen_US
dc.titleEffect of different cryoprotectants (Glycerol, methanol and dimethyl sulfoxide) on post-thaw quality, viability, fertilization ability and dna damage of cryopreserved nile tilapia (oreochromis niloticus) spermatozoaen_US
dc.typeArticleen_US

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