Genomic characterization of pestiviruses isolated from bovine, ovine and caprine foetuses in Turkey: A potentially new genotype of Pestivirus I species

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dc.authoridSevik, Murat/0000-0002-9604-3341
dc.contributor.authorSevik, Murat
dc.date.accessioned2024-09-18T19:48:00Z
dc.date.available2024-09-18T19:48:00Z
dc.date.issued2021
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractThis study was carried out to investigate the frequency and genetic diversity of pestiviruses in abortion cases in cattle and small ruminants in Turkey. During January 2012 and December 2017, a total of 2029 aborted foetuses (553 bovine foetuses, 1,388 sheep foetuses and 88 goat foetuses) were collected from different regions of Turkey. Real-time RT-PCR (RRT-PCR) assays were used to detect pestiviral RNA in aborted foetuses. To confirm the cause of abortion, pestivirus-positive foetuses were also examined for the presence ofBrucellaspp.,Campylobacterspp.,Chlamydophila abortus(C. abortus), akabane virus, bluetongue virus and Schmallenberg virus by molecular detection methods. Pestiviral RNA was detected in 61 (11%) of the 553 bovine foetuses, 124 (8.9%) of the 1,388 sheep foetuses and 3 (3.4%) of the 88 goat foetuses. Furthermore,C. abortusDNA was detected in 3 pestivirus-positive sheep foetuses, whereas other infectious agents were not detected in pestivirus-positive foetuses. Genetic characterization of the pestivirus RRT-PCR positive samples was conducted by sequencing 5 ' untranslated (5 ' UTR) and non-structural autoprotease (N-pro) genomic regions. A total of 68 sequences were obtained, and phylogenetic analyses revealed that all sequences belonged to BVDV-1, including 1b (8/68), 1f (2/68), 1l (4/68), 1r (10/68), Aydin-like pestivirus (20/68) and one unknown genotype (24/68). The 5 ' UTR and N(pro)sequences of this unknown genotype differed from pestiviruses previously described, providing evidence for the presence of an emerging genotype within the species Pestivirus I, tentatively named as 'Konya-like' pestivirus. 'Konya-like' pestivirus was the dominant genotype in sheep foetuses, whereas Aydin-like pestivirus was found to be the predominant genotype in bovine foetuses. To the best my knowledge, this is the first report of Aydin-like pestivirus infection in cattle. The information provided in this study contributes to the understanding the dissemination and evolution of pestiviruses and could be beneficial for developing more effective vaccines.en_US
dc.identifier.doi10.1111/tbed.13691
dc.identifier.endpage426en_US
dc.identifier.issn1865-1674
dc.identifier.issn1865-1682
dc.identifier.issue2en_US
dc.identifier.pmid32564510en_US
dc.identifier.scopus2-s2.0-85087652978en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage417en_US
dc.identifier.urihttps://doi.org/10.1111/tbed.13691
dc.identifier.urihttps://hdl.handle.net/20.500.12483/7283
dc.identifier.volume68en_US
dc.identifier.wosWOS:000545604100001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWiley-Hindawien_US
dc.relation.ispartofTransboundary and Emerging Diseasesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectabortionen_US
dc.subjectcattleen_US
dc.subjectgenetic characterizationen_US
dc.subjectgoatsen_US
dc.subjectpestivirusen_US
dc.subjectsheepen_US
dc.titleGenomic characterization of pestiviruses isolated from bovine, ovine and caprine foetuses in Turkey: A potentially new genotype of Pestivirus I speciesen_US
dc.typeArticleen_US

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