THE GENETIC DIVERSITY OF GENUS BACILLUS AND THE RELATED GENERA REVEALED BY 16S rRNA GENE SEQUENCES AND ARDRA ANALYSES ISOLATED FROM GEOTHERMAL REGIONS OF TURKEY

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dc.authoridColeri Cihan, Arzu/0000-0002-7289-6251
dc.contributor.authorCihan, Arzu Coleri
dc.contributor.authorTekin, Nilgun
dc.contributor.authorOzcan, Birgul
dc.contributor.authorCokmus, Cumhur
dc.date.accessioned2024-09-18T20:28:11Z
dc.date.available2024-09-18T20:28:11Z
dc.date.issued2012
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractPreviously isolated 115 endospore-forming bacilli were basically grouped according to their temperature requirements for growth: the thermophiles (74%), the facultative thermophiles (14%) and the mesophiles (12%). These isolates were taken into 16S rRNA gene sequence analyses, and they were clustered among the 7 genera: Anoxybacillus, Aeribacillus, Bacillus, Brevibacillus, Geobacillus, Paenibacillus, and Thermoactinomycetes. Of these bacilli, only the thirty two isolates belonging to genera Bacillus (16), Brevibacillus (13), Paenibacillus (1) and Thermoactinomycetes (2) were selected and presented in this paper. The comparative sequence analyses revealed that the similarity values were ranged as 91.4-100 %, 91.8- 99.2 %, 92.6- 99.8 % and 90.7 - 99.8 % between the isolates and the related type strains from these four genera, respectively. Twenty nine of them were found to be related with the validly published type strains. The most abundant species was B. thermoruber with 9 isolates followed by B. pumilus (6), B. lichenformis (3), B. subtilis (3), B. agri (3), B. smithii (2), T. vulgaris (2) and finally P. barengoltzii (1). In addition, isolates of A391a, B51a and D295 were proposed as novel species as their 16S rRNA gene sequences displayed similarities <= 97% to their closely related type strains. The AluI-, HaeIII-and TaqI-ARDRA results were in congruence with the 16S rRNA gene sequence analyses. The ARDRA results allowed us to differentiate these isolates, and their discriminative restriction fragments were able to be determined. Some of their phenotypic characters and their amylase, chitinase and protease production were also studied and biotechnologically valuable enzyme producing isolates were introduced in order to use in further studies.en_US
dc.description.sponsorshipFundamental Research Group of The Scientific and Technological Research Council of Turkey (TUBITAK); [TBAG/HD-320 (107T286)]en_US
dc.description.sponsorshipThis study was supported by Fundamental Research Group of The Scientific and Technological Research Council of Turkey (TUBITAK); project number TBAG/HD-320 (107T286). In addition we are very grateful to Prof. Dr. Hakan Akbulut (Ankara University, Faculty of Medicine, Oncology Department, Ankara, Turkey) and Associated Prof. Dr. Hilal Ozdag (Ankara University, Biotechnology Institute Central Laboratory, Genomic Unite, Ankara, Turkey) for their contributions.en_US
dc.identifier.endpage324en_US
dc.identifier.issn1517-8382
dc.identifier.issn1678-4405
dc.identifier.issue1en_US
dc.identifier.pmid24031834en_US
dc.identifier.startpage309en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12483/10778
dc.identifier.volume43en_US
dc.identifier.wosWOS:000303939800037en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofBrazilian Journal of Microbiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectisolationen_US
dc.subjecttemperature requirementen_US
dc.subjectendospore-forming bacillien_US
dc.subject16S rRNA geneen_US
dc.subjectARDRAen_US
dc.titleTHE GENETIC DIVERSITY OF GENUS BACILLUS AND THE RELATED GENERA REVEALED BY 16S rRNA GENE SEQUENCES AND ARDRA ANALYSES ISOLATED FROM GEOTHERMAL REGIONS OF TURKEYen_US
dc.typeArticleen_US

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