Investigation of New Benzimidazole Derivative Compounds' Effects on A549 Cell Line

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dc.authoridTerzi, Menderes Yusuf/0000-0001-8478-0451
dc.contributor.authorDuran, Gulay Gulbol
dc.contributor.authorKucuk, Meral Urhan
dc.contributor.authorAlgul, Oztekin
dc.contributor.authorTerzi, Menderes Yusuf
dc.date.accessioned2024-09-18T19:47:58Z
dc.date.available2024-09-18T19:47:58Z
dc.date.issued2020
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractChronic inflammation is a common indication of several diseases, e.g. asthma, chronic obstructive pulmonary disease (COPD), atherosclerosis, etc. Benzimidazole derivatives are preferable compounds to design new analgesic and anti-inflammatory substances due to their unique biological features. We aimed to investigate the effect of a newly synthesized benzimidazole derivative, ORT-83, on A549 human lung adenocarcinoma cell line. ORT-83 was synthesized, and a non-cytotoxic concentration of ORT-83 on A549 cells was detected with MTT assay. To analyze the anti-inflammatory effect of ORT-83, an inflammatory cell culture model was established by stimulating A549 cell line with IL1-beta (10 ng/ml). After 2 hours of treatment with IL1-beta to induce inflammation, A549 cells were exposed to ORT-83 (0.78 mu g/ml) for 24 hours. Thereafter gene expression analyses were performed with qRT-PCR. We found that ORT-83 significantly suppressed the gene expression levels of the proinflammatory cytokines; IL-6, NFkB, and TNF-alpha. However, the increased levels of IL-10 (2.8 folds) by IL-1 beta induction did not change after ORT-83 and/or dexamethasone (Dex: positive control) treatments. While Dex; a COX-2 inhibitor, reduced the COX-2 expression level in inflammatory cells from 10.03 folds to 0.71 folds, ORT-83 reduced its level to 4.37 folds. iNOS expression levels did not change in any experimental groups. In conclusion, we showed that ORT-83 exerted its anti-inflammatory effects by repressing the gene expression of proinflammatory cytokines in the inflammation-induced A549 cell line. Although ORT-83 had a weaker COX-2 inhibitory effect compared to Dex, it was shown to be still a strong anti-inflammatory compound.en_US
dc.description.sponsorshipHatay Mustafa Kemal University [15704]en_US
dc.description.sponsorshipThis project was supported by Hatay Mustafa Kemal University Scientific Research Projects Funding with project number 15704.en_US
dc.identifier.doi10.1590/1678-4324-2020190364
dc.identifier.issn1516-8913
dc.identifier.issn1678-4324
dc.identifier.scopus2-s2.0-85090269549en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1590/1678-4324-2020190364
dc.identifier.urihttps://hdl.handle.net/20.500.12483/7265
dc.identifier.volume63en_US
dc.identifier.wosWOS:000568669100001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherInst Tecnologia Paranaen_US
dc.relation.ispartofBrazilian Archives of Biology and Technologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectA549en_US
dc.subjectbenzimidazoleen_US
dc.subjectinflammationen_US
dc.subjectanti-inflammatoryen_US
dc.titleInvestigation of New Benzimidazole Derivative Compounds' Effects on A549 Cell Lineen_US
dc.typeArticleen_US

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