Assessment of replication of bovine herpesvirus type 4 in human glioblastoma and breast cancer cells as a potential oncolytic virus

Basit Öğe Kaydı
dc.authoridAlkan, Feray/0000-0003-3854-6503
dc.authoridOzkul, Aykut/0000-0001-5008-9443
dc.contributor.authorAligholipour Farzani, Touraj
dc.contributor.authorBilge Dagalp, Seval
dc.contributor.authorOzkul, Aykut
dc.contributor.authorGurdal, Hakan
dc.contributor.authorDogan, Firat
dc.contributor.authorAlkan, Feray
dc.date.accessioned2024-09-18T20:04:38Z
dc.date.available2024-09-18T20:04:38Z
dc.date.issued2021
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractOncolytic viruses have been extensively used in cancer treatment due to their tropism, selective replication only in tumor cells, and possible synergic interaction with other therapeutics. Different researchers have demonstrated that bovine herpesvirus 4 (BoHV-4), a member of the gammaherpesviridae family, has oncolytic potential in some human-origin cancer cell lines like glioma through the selective replication strategy. Using four apoptosis detection methods, namely MTT, LDH, TUNEL, and Annexin V assays, we evaluated the apoptotic effect of BoHV-4 Movar33/63 reference strain along with a recombinant BoHV-4 expressing EGFP in U87 MG cells (human glioblastoma cell line), MDA MB-231 (human breast cancer cell line), and MCF10a (non-tumorigenic human mammary epithelial cell line). Our findings indicate that this virus can replicate and induce apoptosis in these cell lines and hinder in vitro proliferation in a dose-dependent manner. In conclusion, BoHV-4 has in vitro potential as a novel oncolytic virus in human cancer therapy. However, its replication potential in the MCF10a cells as a non-tumorigenic human mammary epithelial cell line is a concern in using this virus in cancer therapy, at least against human mammary tumors. Further studies must therefore be conducted to examine the specific apoptotic pathways induced by this virus to move on to further experiments.en_US
dc.description.sponsorshipscientific research projects Ankara University (BAP) [17B0239004]en_US
dc.description.sponsorshipThis study was partially supported by a grant from scientific research projects Ankara University (BAP: Project Number: 17B0239004).en_US
dc.identifier.doi10.1007/s11262-020-01802-z
dc.identifier.endpage39en_US
dc.identifier.issn0920-8569
dc.identifier.issn1572-994X
dc.identifier.issue1en_US
dc.identifier.pmid33104955en_US
dc.identifier.scopus2-s2.0-85093919953en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage31en_US
dc.identifier.urihttps://doi.org/10.1007/s11262-020-01802-z
dc.identifier.urihttps://hdl.handle.net/20.500.12483/8290
dc.identifier.volume57en_US
dc.identifier.wosWOS:000583947200001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofVirus Genesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBoHV-4en_US
dc.subjectReplicationen_US
dc.subjectGlioblastomaen_US
dc.subjectCytopathic effecten_US
dc.subjectHuman breast canceren_US
dc.titleAssessment of replication of bovine herpesvirus type 4 in human glioblastoma and breast cancer cells as a potential oncolytic virusen_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
[ N/A ]
İsim:
Tam Metin / Full text
Boyut:
1.24 MB
Biçim:
Adobe Portable Document Format
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu