Investigation and Molecular Characterization of Colistin Resistance in Commensal Escherichia coli Strains Isolated from Broiler Flocks

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dc.contributor.authorAslantas, O.
dc.contributor.authorKucukaltay, K.
dc.date.accessioned2024-09-18T20:06:14Z
dc.date.available2024-09-18T20:06:14Z
dc.date.issued2023
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractThe emergence and dissemination of plasmid-mediated colistin resistance pose a serious threat to global public health. In this study, a total of 454 commensal Escherichia coli strains isolated from cloacal swabs taken from broiler flocks between 2013 and 2014 were first screened for phenotypic colistin resistance by the broth microdilution method. Twelve phenotypically colistin-resistant isolates were screened for mcr-1 to mcr-5 genes by multiplex PCR. Five of these isolates were found to carry the mcr-1 by PCR. Whole-genome sequencing of the mcr-1 carrying isolates revealed the presence of additional antimicrobial resistance genes and chromosomal gyrA and parC mutations conferring resistance to fluoroquinolones. Analysis of virulence gene content revealed 5-20 virulence genes in the isolates and two isolates were defined as avian enteropathogenic E. coli (APEC). Phylogenetic analysis based on whole-genome and multilocus sequence typing revealed that the strains were closely related to mcr-1 carrying isolates from chicken and human clinical isolates previously reported from different parts of the World. The findings further emphasize the urgent need to implement effective control programs to prevent the emergence and dissemination of colistin-resistant bacteria.en_US
dc.identifier.endpage35en_US
dc.identifier.issn0334-9152
dc.identifier.issn2304-8859
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85171754094en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage25en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12483/8397
dc.identifier.volume78en_US
dc.identifier.wosWOS:001104819900005en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherIsrael Veterinary Medical Assocen_US
dc.relation.ispartofIsrael Journal of Veterinary Medicineen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBroileren_US
dc.subjectColistin resistanceen_US
dc.subjectEscherichia colien_US
dc.subjectmcr-1en_US
dc.subjectWGSen_US
dc.titleInvestigation and Molecular Characterization of Colistin Resistance in Commensal Escherichia coli Strains Isolated from Broiler Flocksen_US
dc.typeArticleen_US

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