Formation of wheat (Triticum aestivum L.) embryogenic callus involves peroxide-generating germin-like oxalate oxidase

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dc.authorscopusid7005185601
dc.authorscopusid6508065901
dc.authorscopusid6701491865
dc.contributor.authorCaliskan, Mahmut
dc.contributor.authorTuret, Müge
dc.contributor.authorCuming, Andrew C.
dc.date.accessioned2024-09-19T15:45:28Z
dc.date.available2024-09-19T15:45:28Z
dc.date.issued2004
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractIn wheat (Triticum aestivum L.), embryogenic callus formation comprises suppression of precocious germination by the zygotic embryo and the initiation of dedifferentiated cellular proliferation within it. Embryogenic calli are induced by treating immature embryos with 2,4-dichlorophenoxyacetic acid (2,4-D). Upon withdrawal from 2,4-D, somatic embryos develop from the periphery of the callus. Prior to visible callus formation, there is a striking induction of "germin-like" oxalate oxidase ("gl-OXO": EC 1.2.3.4) gene expression. Accumulation of gl-OXO mRNA is rapidly stimulated upon auxin treatment, with a consequent development of apoplastic enzyme activity producing H2O2 within the cell wall. Within the dedifferentiated calli, gl-OXO enzyme activity becomes widespread over the surface of embryogenic calli. Differentiation of somatic embryos is initiated in regions of densely cytoplasmic, meristematic cells that are marked by highly localised expression of gl-OXO activity within these embryogenic cell masses. We suggest that this localised generation of H2O2 by gl-OXO promotes peroxidative cross-linking of cell wall components, thereby preventing cellular expansion and maintaining these cell masses in an embryogenically competent condition. © Springer-Verlag 2004.en_US
dc.description.sponsorshipTürkiye Bilimsel ve Teknolojik Araştirma Kurumu, TÜBITAK; Mustafa Kemal Üniversitesien_US
dc.identifier.doi10.1007/s00425-003-1199-9
dc.identifier.endpage140en_US
dc.identifier.issn0032-0935
dc.identifier.issue1en_US
dc.identifier.pmid14745556en_US
dc.identifier.scopus2-s2.0-2442515304en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage132en_US
dc.identifier.urihttps://doi.org/10.1007/s00425-003-1199-9
dc.identifier.urihttps://hdl.handle.net/20.500.12483/14703
dc.identifier.volume219en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.relation.ispartofPlantaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCell wallen_US
dc.subjectEmbryogenic callusen_US
dc.subjectGerminen_US
dc.subjectHydrogen peroxideen_US
dc.subjectOxalate oxidaseen_US
dc.subjectTriticum embryoen_US
dc.titleFormation of wheat (Triticum aestivum L.) embryogenic callus involves peroxide-generating germin-like oxalate oxidaseen_US
dc.typeArticleen_US

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