Spatial localization of germin-like oxalate oxidase genes in developing wheat shoots

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dc.authorscopusid7005185601
dc.authorscopusid6603825851
dc.authorscopusid7006237943
dc.authorscopusid6701491865
dc.contributor.authorÇalişkan, M.
dc.contributor.authorOzcan, B.
dc.contributor.authorTuran, C.
dc.contributor.authorCuming, A.C.
dc.date.accessioned2024-09-19T15:48:48Z
dc.date.available2024-09-19T15:48:48Z
dc.date.issued2004
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractWhen quiescent embryos in dry grains of ripened wheat are isolated and provided with ample water and oxygen at an appropriate temperature, they begin to grow very rapidly. The onset of the growth is signaled by nascent synthesis of germin genes which encodes a relatively rare, water-soluble homop entameric glycoprotein. Germin is resistant to pepsin digestion under conditions that lead to hydrolysis of virtually all other proteins in wheat embryos. Germin proteins have oxalate oxidase activity, an activity that degrades oxalic acid to generate hydrogen peroxide which involves in many aspects of plant development. Following 48-hour imbibition on water, wheat embryos give rise to distinguishable shoot and roots. Shoots comprise coleoptile, leaf primordium and shoot apex. In the current study, non-radioactively labeled germin riboprobes were prepared by in vitro transcription. The riboprobes were used to search and localize germin mRNAs in sections taken from throughout of shoots. The results revealed that although leaf primordium and shoot apex did not show any signals of the presence of germin mRNAs, coleoptiles as a whole tissue displayed germin gene expression on epidermal cells and vascular bundle sheath cells. Among the sections taken from different parts of shoots, the sections from middle part gave the strongest signals on coleoptile cells. © 2004 Taylor and Francis Group, LLC.en_US
dc.identifier.doi10.1080/13102818.2004.10819222
dc.identifier.endpage14en_US
dc.identifier.issn1310-2818
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-1242269197en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage10en_US
dc.identifier.urihttps://doi.org/10.1080/13102818.2004.10819222
dc.identifier.urihttps://hdl.handle.net/20.500.12483/15311
dc.identifier.volume18en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.relation.ispartofBiotechnology and Biotechnological Equipmenten_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectTriticum aestivumen_US
dc.subjectenzymeen_US
dc.subjectgene producten_US
dc.subjectgerminen_US
dc.subjectglycoproteinen_US
dc.subjecthydrogen peroxideen_US
dc.subjectmessenger RNAen_US
dc.subjectoxalate oxidaseen_US
dc.subjectpepsin Aen_US
dc.subjectunclassified drugen_US
dc.subjectarticleen_US
dc.subjectcoleoptileen_US
dc.subjectcontrolled studyen_US
dc.subjectcotyledonen_US
dc.subjectenzyme activityen_US
dc.subjectgeneen_US
dc.subjectgene locationen_US
dc.subjectgermin like oxalate oxidase geneen_US
dc.subjectgerminationen_US
dc.subjecthydrolysisen_US
dc.subjectisotope labelingen_US
dc.subjectnonhumanen_US
dc.subjectplant cellen_US
dc.subjectplant developmenten_US
dc.subjectplant geneticsen_US
dc.subjectplant growthen_US
dc.subjectshooten_US
dc.subjectwater absorptionen_US
dc.subjectwheaten_US
dc.titleSpatial localization of germin-like oxalate oxidase genes in developing wheat shootsen_US
dc.typeArticleen_US

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