Protective Effects of Melatonin on Testicular Torsion and Detorsion Damage in Sprague-Dawley Rats

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dc.authoridTuncer, Mehmet Cudi/0000-0001-7317-5467
dc.contributor.authorAktas, Ayfer
dc.contributor.authorTuncer, M. Cudi
dc.contributor.authorYildirim, Ayse
dc.contributor.authorNergiz, Yusuf
dc.contributor.authorAkkus, Murat
dc.date.accessioned2024-09-18T20:59:20Z
dc.date.available2024-09-18T20:59:20Z
dc.date.issued2011
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractIn this study, we evaluated the ultrastructural findings of testis with systemic administration of different doses of melatonin during ischemic period in a rat model of testicular torsion/detorsion (T/D). Testis ischemia-reperfusion (I/R) injury was induced by torsion of the left testis, with a 720 degrees twisting of the spermatic cord so as to produce a total occlusion of testis for 2.5 hours. Subsequently, the same testis was then detorsioned. According to surgical procedure in each group, unilateral orchiectomies were performed for histopathologic examination. The groups were labelled as control group, torsion group (T), torsion and detorsion group (T/D), torsion-detorsion and melatonin group (T/D+20,50 and 100 mg/kg melatonin). For the histological examination, testicular tissues were fixed in 2.5% glutheraldehyde and postfixation 1% osmic acid solutions. They were examined under transmission electron microscopy after application of contrast stained. In torsion group testis cross-sections, cytoplasm residues of mature sperms and large vacuole-like structures were noticeable. In detorsion group testis cross-sections, dissociations in spermatocide nuclei, many vacuoles and residual particles resulting from organelle degeneration, local voids in cytoplasms of spermatogonia, dilatation in granulated endoplasmic reticulum, large lipid droplets, chromatid particles, along with mitochondrial crystalisis were determined. In the testis cross-sections of the group of T/D+50 mg/kg melatonin administration, sertoli and spermatogonia cells that showed membrane-like structures and cytoplasmic voids were observed. Testis cross-sections of rats that were administered with T/D+50 mg/kg melatonin showed small mitochondrions and vacuole-like structures placed on the edge. Testis cross-sections of rats that were administered with T/D+100 mg/kg melatonin resulted in views similar to those of controls in the microstructural level. As a result, the most effective dose of melatonin, which was used in different doses, for prevention of ischemia/reperfusion damage was found to be 100 mg/kg.en_US
dc.identifier.doi10.4067/S0717-95022011000100001
dc.identifier.endpage15en_US
dc.identifier.issn0717-9502
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-79957505130en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage7en_US
dc.identifier.urihttps://doi.org/10.4067/S0717-95022011000100001
dc.identifier.urihttps://hdl.handle.net/20.500.12483/12527
dc.identifier.volume29en_US
dc.identifier.wosWOS:000290913400001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSoc Chilena Anatomiaen_US
dc.relation.ispartofInternational Journal of Morphologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectTestisen_US
dc.subjectTorsionen_US
dc.subjectDetorsionen_US
dc.subjectSpraque-Dawley raten_US
dc.subjectMelatoninen_US
dc.titleProtective Effects of Melatonin on Testicular Torsion and Detorsion Damage in Sprague-Dawley Ratsen_US
dc.typeArticleen_US

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