Isolation and Molecular Characterization of ESBL/pAmpC Producing Escherichia coli in Household Pigeons in Turkey
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Date
2018
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Israel Veterinary Medical Assoc
Access Rights
info:eu-repo/semantics/closedAccess
Abstract
In this study it was aimed to determine the prevalence of extended-spectrum beta-lactamase (ESBL) and/or plasmidic AmpC beta-lactamase (pAmpC) producing Escherichia coli among household pigeons in Hatay, Turkey. For this purpose, 150 cloacal swab samples were taken from apperently healthy household pigeons and the isolates were characterised by phylogenetic grouping, plasmid replicon typing, pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) analysis. ESBL producing E. coli was detected in five (3.3%) of the cloacal swabs: all the strains carried the bla(CTX-M-15) gene together with bla(TEM-1) (four phylogenetic group B1 and ST446 and one phylogenetic group A and ST665). pAmpC producing E. coli was detected in one (0.7%) isolate and this isolate harbored the bla(CMY-2) (phylogroup B2 and ST4906). Three plasmid replicon types detected among the strains IncII/I., IncFIB and IncFrepB were the most common. Three distinct PFGE pulsotypes were found among the isolates and multidrug resistance (MDR) phenotype was only observed in two isolates. The current study was, to the best knowledge of the authors, the first showing that household pigeons in Turkey are fecal carries of ESBL/pAmpC producing E. coli and a significant potential source of these bacteria for humans.
Description
Keywords
Pigeon, E. coli, ESBL, pAmpC, MLST, PFGE, Plasmid Replicon Typing
Journal or Series
Israel Journal of Veterinary Medicine
WoS Q Value
Q4
Scopus Q Value
Q4
Volume
73
Issue
4
