Identification and Characterization of a Novel Robigovirus Species from Sweet Cherry in Turkey

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dc.authoridRoumi, Vahid/0000-0002-6496-2298
dc.contributor.authorCaglayan, Kadriye
dc.contributor.authorRoumi, Vahid
dc.contributor.authorGazel, Mona
dc.contributor.authorElci, Eminur
dc.contributor.authorAcioglu, Mehtap
dc.contributor.authorPlesko, Irena Mavric
dc.contributor.authorReynard, Jean-Sebastien
dc.date.accessioned2024-09-18T20:55:30Z
dc.date.available2024-09-18T20:55:30Z
dc.date.issued2019
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractHigh throughput sequencing of total RNA isolated from symptomatic leaves of a sweet cherry tree (Prunus avium cv. 0900 Ziraat) from Turkey identified a new member of the genus Robigovirus designated cherry virus Turkey (CVTR). The presence of the virus was confirmed by electron microscopy and overlapping RT-PCR for sequencing its whole-genome. The virus has a ssRNA genome of 8464 nucleotides which encodes five open reading frames (ORFs) and comprises two non-coding regions, 5 ' UTR and 3 ' UTR of 97 and 296 nt, respectively. Compared to the five most closely related robigoviruses, RdRp, TGB1, TGB2, TGB3 and CP share amino acid identities ranging from 43-53%, 44-60%, 39-43%, 38-44% and 45-50%, respectively. Unlike the four cherry robigoviruses, CVTR lacks ORFs 2a and 5a. Its genome organization is therefore more similar to African oil palm ringspot virus (AOPRV). Using specific primers, the presence of CVTR was confirmed in 15 sweet cherries and two sour cherries out of 156 tested samples collected from three regions in Turkey. Among them, five samples were showing slight chlorotic symptoms on the leaves. It seems that CVTR infects cherry trees with or without eliciting obvious symptoms, but these data should be confirmed by bioassays in woody and possible herbaceous hosts in future studies.en_US
dc.description.sponsorshipTUBITAK-TOVAG [213O042]; Slovenian Research Agency [P4-0072]; COST Action [FA1407]en_US
dc.description.sponsorshipThis work was supported by TUBITAK-TOVAG grant number 213O042, the Slovenian Research Agency grant P4-0072 and by the COST Action grant number FA1407 (DIVAS).en_US
dc.identifier.doi10.3390/pathogens8020057
dc.identifier.issn2076-0817
dc.identifier.issue2en_US
dc.identifier.pmid31035571en_US
dc.identifier.scopus2-s2.0-85068536171en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.3390/pathogens8020057
dc.identifier.urihttps://hdl.handle.net/20.500.12483/11880
dc.identifier.volume8en_US
dc.identifier.wosWOS:000475287800016en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMdpien_US
dc.relation.ispartofPathogensen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectPrunus aviumen_US
dc.subjecthigh throughput sequencingen_US
dc.subjectBetaflexiviridaeen_US
dc.subjectcherry virus Turkeyen_US
dc.titleIdentification and Characterization of a Novel Robigovirus Species from Sweet Cherry in Turkeyen_US
dc.typeArticleen_US

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