Assessing the effects of the neonicotinoid insecticide imidacloprid in the cholinergic synapses of the stellate cells of the mouse cochlear nucleus using whole-cell patch-clamp recording

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dc.authoridNAZIROGLU, Mustafa/0000-0003-0887-6974
dc.authoridBal, Ramazan/0000-0003-3829-8669
dc.authoridBaydas, Giyasettin/0000-0002-9206-3177
dc.contributor.authorBal, Ramazan
dc.contributor.authorErdogan, Suat
dc.contributor.authorTheophilidis, George
dc.contributor.authorBaydas, Giyasettin
dc.contributor.authorNaziroglu, Mustafa
dc.date.accessioned2024-09-18T20:27:57Z
dc.date.available2024-09-18T20:27:57Z
dc.date.issued2010
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractImidacloprid (IMI) is widely used systemic insecticide that acts as an agonist on nicotinic acetylcholine receptors (nAChRs). IMI has been reported to be more active against insect nAChRs (EC(50) 0.86-1 mu M) than it is against mammalian nAChRs (EC(50) 70 mu M). The objective of this study was to determine to what extent IMI affects the nAChRs of the stellate cells of mouse cochlear nucleus (CN), using whole-cell patch-clamp recording. Puff application of 1 mu M IMI had no significant effect on the membrane properties of the neurons tested, while a concentration of 10 mu M caused a significant depolarizing shift in the membrane potential and resulted in increases in the fluctuation of the membrane potential and in the frequency of miniature postsynaptic potentials (mpps) within less than a minute of exposure. IMI at concentrations >= 50 mu M caused a significant depolarizing shift in the membrane potential, accompanied by a marked increase in the frequency of action potential. IMI decreased the membrane input resistance and the membrane time constants. Bath application of 50 mu M d-tubocurarine (d-TC) reversibly blocked the depolarizing shift of the resting membrane potential and the spontaneous firing induced by IMI application in Current clamp and blocked the inward Currents through nicotinic receptors induced by IMI application in voltage clamp. Similarly, 100 nM alpha-bungarotoxin (alpha-BgTx) blocked the spontaneous firing induced by IMI (n = 3). The amplitude of the 100 mu M IMI-induced inward current at -60 mV holding potential was 115.0 +/- 16.2 pA (n = 7). IMI at a concentration of 10 mu M produced 11.3 +/- 3.4 pA inward current (n = 4). We conclude that exposure to IMI at concentrations >= 10 mu M for <1 min can change the membrane properties of neurons that have nAChRs and. as a consequence, their function. (C) 2009 Elsevier Inc. All rights reserved.en_US
dc.description.sponsorshipScientific and Technical Research Council of Turkey (TUBITAK) (Turkey) [1050705]; Ministry for Development, General Secretariat for Research Technology (GSRT) (Greece)en_US
dc.description.sponsorshipThis work was supported by a joint grant from The Scientific and Technical Research Council of Turkey (TUBITAK) (Project no: 1050705) (Turkey) and The Ministry for Development, General Secretariat for Research Technology (GSRT) (Greece).en_US
dc.identifier.doi10.1016/j.neuro.2009.10.004
dc.identifier.endpage120en_US
dc.identifier.issn0161-813X
dc.identifier.issue1en_US
dc.identifier.pmid19853623en_US
dc.identifier.scopus2-s2.0-74249102208en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage113en_US
dc.identifier.urihttps://doi.org/10.1016/j.neuro.2009.10.004
dc.identifier.urihttps://hdl.handle.net/20.500.12483/10638
dc.identifier.volume31en_US
dc.identifier.wosWOS:000274611000012en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofNeurotoxicologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectImidaclopriden_US
dc.subjectNeonicotinoidsen_US
dc.subjectToxicityen_US
dc.subjectNicotinic receptoren_US
dc.subjectPatch clampen_US
dc.subjectElectrophysiologyen_US
dc.titleAssessing the effects of the neonicotinoid insecticide imidacloprid in the cholinergic synapses of the stellate cells of the mouse cochlear nucleus using whole-cell patch-clamp recordingen_US
dc.typeArticleen_US

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