The protective effects of alpha lipoic acid on methotrexate induced testis injury in rats

Basit Öğe Kaydı
dc.contributor.authorPinar, Neslihan
dc.contributor.authorCakirca, Gokhan
dc.contributor.authorOzgur, Tumay
dc.contributor.authorKaplan, Mahir
dc.date.accessioned2024-09-18T20:32:49Z
dc.date.available2024-09-18T20:32:49Z
dc.date.issued2018
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractMethotrexate (MTX) is frequently used in the treatment of several diseases including cancers, rheumatoid arthritis, psoriatic arthritis, systemic lupus erythematosus, and dermatomyositis. Previously, chemotherapeutic agents have been reported to cause permanent azoospermia and infertility in men. Methotrexate has been also shown to damage the seminiferous tubules of the testicles, lower the sperm count, and cause genetic mutations (in DNA) in sperm. In this study, we aimed to investigate the protective effects of alpha lipoic acid (ALA) on MTX-induced testicle damage in a rat model. A total of 40 male Wistar Albino rats were used in this study. The rats were divided into four groups including 10 rats in each. The first group (control group) received only saline intraperitoneal (i.p.); the second group (ALA group) was given ALA 100 mg/kg i.p.; the third group (MTX group) received single dose MTX 20 mg/kg i.p.; and the fourth group (MTX+ALA group) received single dose MTX 20 mg/kg i.p. and ALA 100 mg/kg i.p. Malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), myeloperoxidase (MPO) levels in the testicular tissue and serum testosterone, serum total antioxidant status (TAS) and total oxidant status (TOS) levels were biochemically evaluated. Testicular tissues histopathologically evaluated. In the MTX group, the MDA, TAS and TOS levels were higher, while the SOD, CAT, GPx, MPO and serum testosterone levels decreased. Compared to the MTX group, the MDA, TAS and TOS levels were lower and the SOD, CAT, GPx, MPO and serum testosterone levels increased in the MTX+ALA group. In the histopathological examination, the mean seminiferous tubule length (MSTD), germinal epithelial cell thickness (GECT), and mean testicular biopsy score (MTBS) were found to significantly decrease in the MTX group, compared to the control group. These values were significantly higher in the MTX+ ALA group, compared to the MTX group (p < 0.05). In our experimental study, MTX caused severe tissue destruction in testicles by increasing the formation of free oxygen radicals. Based on our study results, we suggest that, as a potent free radical scavenger, ALA can reduce MTX-induced testicular tissue damage thanks to its antioxidant and anti-inflammatory properties.en_US
dc.description.sponsorshipScientific Research Office of Mustafa Kemal University [16345/2016]en_US
dc.description.sponsorshipThis work was supported by the Scientific Research Office of Mustafa Kemal University (Project no: 16345/2016).en_US
dc.identifier.doi10.1016/j.biopha.2017.11.078
dc.identifier.endpage1492en_US
dc.identifier.issn0753-3322
dc.identifier.issn1950-6007
dc.identifier.pmid29793311en_US
dc.identifier.scopus2-s2.0-85036658532en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1486en_US
dc.identifier.urihttps://doi.org/10.1016/j.biopha.2017.11.078
dc.identifier.urihttps://hdl.handle.net/20.500.12483/11151
dc.identifier.volume97en_US
dc.identifier.wosWOS:000419041300179en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier France-Editions Scientifiques Medicales Elsevieren_US
dc.relation.ispartofBiomedicine & Pharmacotherapyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMethotrexateen_US
dc.subjectTestis injuryen_US
dc.subjectAlpha lipoic aciden_US
dc.titleThe protective effects of alpha lipoic acid on methotrexate induced testis injury in ratsen_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
[ N/A ]
İsim:
Tam Metin / Full Text
Boyut:
1.19 MB
Biçim:
Adobe Portable Document Format
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu