Hyperosmolarity induced cystine transport and cystine-cysteine cycle between erythrocytes and the plasma

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dc.contributor.authorYildiz, Deniz
dc.contributor.authorCakir, Yeliz
dc.date.accessioned2024-09-18T20:14:59Z
dc.date.available2024-09-18T20:14:59Z
dc.date.issued2008
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractThe objective of the present study was to determine if a cystine-cysteine cycle operates between the erythrocytes and the plasma. In the present study we incubated the erythrocytes in krebs ringer phosphate buffer with different osmolarity containing different amounts of cystine. Our results show that erythrocytes do not uptake cystine from the environment when the osmolarity of the buffer is 310mOsmol/l.Erythrocytes also do not operate a cystine-cysteine cycle in this isoosmolar buffer. However, when exposed to hyperosmolar buffer in the ranges that occur in the kidney medulla which is in between 1200-1400 mOsmol/l erythrocytes start to uptake cystine from the environment and induce a cystine-cysteine cycle. The cystine uptake and cystine-cysteine cycle were characterized by measurement of changes in the free -SH concentrations in erythrocytes and in the buffer. Following incubation of erythrocytes in 1 mM cystine containing 1250 and 1300 mOsmol/l buffer, the free -SH concentrations in the buffer reached to 0. 102 +/- 0.002 and 0.241 +/- 0.013 mu mol/ml erythrocyte respectively. Our results demonstrate that erythrocytes display a cystine-cysteine cycle in hyperosmolar environment which is prevailed mainly in the kidney medulla. Our results also display that this process is biologically active and energy dependent. The observed cystine-cysteine cycle is inhibited when the erythrocytes are incubated at lower temperatures and in the absence of glucose. Our results suggest that erythrocytes uptake cystine, intracellulary reduce it to cysteine and release it back to the environment when exposed to hyperosmolar conditions. Erythrocytes may have a role in the regulation of plasma cystine and cysteine concentrations and may contribute to the regulation of plasma redox status.en_US
dc.identifier.doi10.1248/jhs.54.187
dc.identifier.endpage195en_US
dc.identifier.issn1344-9702
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-44449146445en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage187en_US
dc.identifier.urihttps://doi.org/10.1248/jhs.54.187
dc.identifier.urihttps://hdl.handle.net/20.500.12483/9355
dc.identifier.volume54en_US
dc.identifier.wosWOS:000255510600008en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherPharmaceutical Soc Japanen_US
dc.relation.ispartofJournal of Health Scienceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectcystine-cysteine cycleen_US
dc.subjecthyperosmolarityen_US
dc.subjecterythrocytesen_US
dc.titleHyperosmolarity induced cystine transport and cystine-cysteine cycle between erythrocytes and the plasmaen_US
dc.typeArticleen_US

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