Molecular Characterization of Echinococcus granulosus Isolates Obtained From Different Hosts

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dc.contributor.authorErdogan, Emrah
dc.contributor.authorOzkan, Bora
dc.contributor.authorMutlu, Fatih
dc.contributor.authorKaraca, Serkan
dc.contributor.authorSahin, Izzet
dc.date.accessioned2024-09-18T20:14:59Z
dc.date.available2024-09-18T20:14:59Z
dc.date.issued2017
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractEchinococcus granulosus is a parasite that can be seen throughout the world. So far, five species of genus Echinococcus have been identified as parasite in people: E.granulosus, E.multilocularis, E.vogeli, E.oligarthrus, E.shiquicus. Larval (metacestod) form of parasite settles in internal organs of hoofed animals (cattle, goats, pigs, horses, sheep, etc.) and human; the adult form is found in small intestine of final host, canine. Disease caused by parasite called as Cystic echinococcosis (CE) is an important health problem and causes economic losses in many countries including our country that livestock is common. Infective eggs cause infections in intermediate hosts by taking oral way and rarely inhalation. Received egg opens in the stomach and intestines of intermediate host and oncosphere is released. Oncosphere quickly reaches the lamina propria of the villus epithelium by its histolytic enzymes and hooks. It usually transported from here to the liver and lungs, less frequently, muscle, brain, spleen, kidney and to other organs through the veins. By molecular studies, five species have been validated taxonomically and 10 different variants or strains of E.granulosus have been identified. Host and developmental differences between strains may negatively affect control studies and fight against the parasite. This study aimed to determinate E.granulosus strains obtained from cyst material of different intermediate hosts from different regions of Turkey by molecular methods. In the study, 25 human, 8 cattle, 6 sheep and 2 goat cysts material has been collected. Total genomic DNA was isolated from protoscoleces in cyst fluid and analyzed by PCR with COX-1 (L) and COX-1 (S) genes specific primers. DNA sequence analysis for each PCR product has been made. DNA sequence analysis results evaluated phylogenetically by MEGA analyze and BLAST software. As a result of this study, all isolates were identified as E.granulosus sensu stricto (G1) by DNA sequence analysis. CE is a major public health problem for our country so we believe that obtained data from this study is an important source for parasite control, effective diagnosis, treatment techniques, eradication, vaccination and drug development. Similar studies will be beneficial to cover all other regions of Turkey and to develop effective and successful control programs.en_US
dc.identifier.doi10.5578/mb.45452
dc.identifier.endpage86en_US
dc.identifier.issn0374-9096
dc.identifier.issue1en_US
dc.identifier.pmid28283013en_US
dc.identifier.scopus2-s2.0-85018916148en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage79en_US
dc.identifier.urihttps://doi.org/10.5578/mb.45452
dc.identifier.urihttps://hdl.handle.net/20.500.12483/9354
dc.identifier.volume51en_US
dc.identifier.wosWOS:000396551000009en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isotren_US
dc.publisherAnkara Microbiology Socen_US
dc.relation.ispartofMikrobiyoloji Bultenien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCystic echinococcosisen_US
dc.subjectEchinococcus granulosusen_US
dc.subjectstrainsen_US
dc.subjectmolecular characterizationen_US
dc.titleMolecular Characterization of Echinococcus granulosus Isolates Obtained From Different Hostsen_US
dc.typeArticleen_US

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