Effect of different plant extracts on microbial population in the frozen African Catfish (Clarias gariepinus) semen
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Dosyalar
Tarih
2023
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
C M B Assoc
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Fish sperm cryopreservation has been attempted on roughly freshwater and marine species since 1953. This study sought to assess the potential of various plant extracts to function as natural antimicrobial agents in the frozen semen of African catfish (Clarias gariepinus). Diluted sperm was packaged in 0.25ml straws and left for 10min equilibration at 4 degrees C. Following equilibration, the straws were exposed to liquid nitrogen vapor for 10 min and plunged into the liquid nitrogen (-196 degrees C) and then thawed in a water bath at 35 degrees C for 20s. Sperm samples were put into sterile 1.5 ml tubes immediately after thawing and the microbial count was detected with classical microbiological culture method. In the results of microbiological analyses, these tree plant extracts especially Echinacea purpurea were found highly effective for decreasing bacterial contamination levels of African catfish (C. gariepinus) semen. These plant extracts may have the potential for antibacterial effect, and they can be useful for the dilution of semen.
Açıklama
Anahtar Kelimeler
African catfish, bacterial count, Clarias gariepinus, plant extract, semen
Kaynak
Cellular and Molecular Biology
WoS Q Değeri
Q4
Scopus Q Değeri
Q4
Cilt
69
Sayı
8
