Prevalence of ?-Lactamase Producing Escherichia coli from Retail Meat in Turkey

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dc.contributor.authorOnen, Sevda Pehlivanlar
dc.contributor.authorAslantas, Ozkan
dc.contributor.authorYilmaz, Ebru Sebnem
dc.contributor.authorKurekci, Cemil
dc.date.accessioned2024-09-18T20:04:21Z
dc.date.available2024-09-18T20:04:21Z
dc.date.issued2015
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractExtended spectrum beta-lactamase (ESBL) and plasmid-mediated AmpC beta-lactamase (pAmpC) producing Escherichia coli have been shown to be present in humans and animals representing a significant problem worldwide. This study aimed to search the presence of ESBL and/or AmpC-producing E. coli in retail meats (chicken and beef) in Turkey. A total of 88 beta-lactamase-producing E. coli were isolated from chicken (n = 81/100) and beef meat (n = 7/100) samples and their susceptibility to several antimicrobials were tested using disc diffusion method. E. coli isolates were further characterized for their phylogenetic groups. beta-Lactamase encoding (bla(TEM), bla(SHV), bla(OXA), bla(CTX-M), and bla(AmpC)) and quinolone resistance genes (qnrA, qnrB, qnrS, qepA, and acc(6')-Ib-cr) were also secreened by polymerase chain reaction (PCR). However, in regard to beta-lactamase genes, 84 of 88 isolates were positive for bla(CTX-M-1) (n = 39), bla(CTX-M-3) (n = 5), bla(CTX-M-15) (n = 4), bla(TEM-1b) (n = 2), bla(SHV-12) (n = 1), bla(CTX-M-1/blaTEM-1b) (n = 10), bla(CTX-M-1)/bla(TEM-1b)/bla(SHV-5) (n = 1), bla(CTX-M-1)/bla(CMY-2) (n = 1) and bla(TEM-1b)/bla(CMY-2) (n = 6), bla(CTX-M-15)/bla(SHV-12) (n = 1), bla(CTX-M-15)/bla(TEM-1b) (n = 1), bla(TEM-1b)/bla(SHV-12) (n = 1), and bla(CMY-2) (n = 12) genes. Resistance to cefuroxime (75.6% and 85.7%), nalidixic acid (89% and 85.7%), tetracycline (91.4% and 100%), streptomycin (40.2% and 100%), and trimethoprim-sulfamethoxazole (36.6% and 85.7%) was observed among strains isolated from chicken and beef, respectively. However, all isolates were found to be susceptible to amikacin, imipenem, and cefepime. Resistance to ampicillin and cefoxitin was significantly linked to bla(CMY-2) gene, while there was a significant correlation between CTX-M type ESBL and antimicrobial resistance to cefuroxime and streptomycin (P < 0.05). The results of this study suggest that raw chicken retail meats are highly contaminated with ESBL-producing E. coli implementing a great risk to human health in Turkey.en_US
dc.description.sponsorshipMustafa Kemal Univ. [BAP-1207 M 0107-2012]en_US
dc.description.sponsorshipThis study received a grant from Mustafa Kemal Univ. (BAP-1207 M 0107-2012). We gratefully thank Dr. George Jacoby (Lahey Hospital and Medical Center, U.S.A.) and Prof. Dr. Patrice Courvalin (Pasteur Institute, France) for the gift of the E. coli strains. We are also thankful to Dr. Rafat Al Jassim (The Univ. of Queensland, Australia) for improving the manuscript.en_US
dc.identifier.doi10.1111/1750-3841.12984
dc.identifier.endpageM2029en_US
dc.identifier.issn0022-1147
dc.identifier.issn1750-3841
dc.identifier.issue9en_US
dc.identifier.scopus2-s2.0-84941742274en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpageM2023en_US
dc.identifier.urihttps://doi.org/10.1111/1750-3841.12984
dc.identifier.urihttps://hdl.handle.net/20.500.12483/8107
dc.identifier.volume80en_US
dc.identifier.wosWOS:000364634300016en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofJournal of Food Scienceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectbeef meaten_US
dc.subjectchicken meaten_US
dc.subjectESBLen_US
dc.subjectE. colien_US
dc.titlePrevalence of ?-Lactamase Producing Escherichia coli from Retail Meat in Turkeyen_US
dc.typeArticleen_US

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