Synthesis, characterization, biological activity and electrochemistry studies of iron(III) complex with curcumin-oxime ligand
| dc.contributor.author | Ozbolat, Guluzar | |
| dc.contributor.author | Yegani, Arash Alizadeh | |
| dc.date.accessioned | 2024-09-18T20:08:05Z | |
| dc.date.available | 2024-09-18T20:08:05Z | |
| dc.date.issued | 2020 | |
| dc.department | Hatay Mustafa Kemal Üniversitesi | en_US |
| dc.description.abstract | Iron overload is a key target in drug development. This study aimed to investigate the coordination of Fe(III) ions with a curcumin-oxime ligand that may be used in the treatment of iron overload. The synthesis of the curcumin-oxime ligand and curcumin-oxime-Fe(III) complex was successfully made and characterized in its solid-state and solution-state using FT-IR, UV-Vis, elemental analysis, and H-1-NMR. However, in this study, we investigated the apoptotic effects of the curcumin-oxime Fe (III) complex on SW480. SW480 cells were exposed to 99.2% medium for 48 hours. After 48 hours, the incubation period, cells were harvested by centrifugation and washed in phosphate-buffered saline (PBS) and lysed in radio-immunoprecipitation assay (RIPA) buffer for 20 minutes and supernatants were taken and pellets were discarded. ELISA test was used to examine the expression, and activity of cleaved caspase-3, Bax, and Bcl-2 proteins in SW480 cells. ELISA test results indicated that the activities of apoptotic proteins Bax, caspase 3 and Bcl-2 in human SW480 cell lines significantly increased in 48 hours treatment. Also, the activity of Bcl-2 was observed to decrease significantly. Catalase activities of the complex were investigated. The findings showed that the complex has a catalase activity. The findings suggest that this type of complex may constitute a new and interesting basis for the future search of new and more potent drugs. The SOD activity of the result showed that the complexes possessed a considerable SOD activity with an IC50 value of 7.685 mu M. Also, when compared with the control, a complex increased the SOD levels (P < .05). Electrochemistry studies in the literature have shown that the Fe3+/Fe(2+)couple redox process occurs in low potential. This value is within the range of compounds that are expected to show superoxide dismutase activity. The I-pc/I(pa)shows that one electron transport takes place in the complex. Our results suggest that curcumin-oxime may represent a new approach in the treatment of iron overload. | en_US |
| dc.identifier.doi | 10.1111/1440-1681.13359 | |
| dc.identifier.endpage | 1842 | en_US |
| dc.identifier.issn | 0305-1870 | |
| dc.identifier.issn | 1440-1681 | |
| dc.identifier.issue | 11 | en_US |
| dc.identifier.pmid | 32497256 | en_US |
| dc.identifier.scopus | 2-s2.0-85089539947 | en_US |
| dc.identifier.scopusquality | Q2 | en_US |
| dc.identifier.startpage | 1834 | en_US |
| dc.identifier.uri | https://doi.org/10.1111/1440-1681.13359 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12483/8614 | |
| dc.identifier.volume | 47 | en_US |
| dc.identifier.wos | WOS:000563877600001 | en_US |
| dc.identifier.wosquality | Q3 | en_US |
| dc.indekslendigikaynak | Web of Science | en_US |
| dc.indekslendigikaynak | Scopus | en_US |
| dc.indekslendigikaynak | PubMed | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | Wiley | en_US |
| dc.relation.ispartof | Clinical and Experimental Pharmacology and Physiology | en_US |
| dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
| dc.rights | info:eu-repo/semantics/closedAccess | en_US |
| dc.subject | catalase | en_US |
| dc.subject | caspase-3 | en_US |
| dc.subject | chelation | en_US |
| dc.subject | curcumin-oxime | en_US |
| dc.subject | iron overload | en_US |
| dc.subject | ligand | en_US |
| dc.title | Synthesis, characterization, biological activity and electrochemistry studies of iron(III) complex with curcumin-oxime ligand | en_US |
| dc.type | Article | en_US |
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