Phylogenetic relationship among taxa in the genus Adonis L. collected from Turkiye based on nrDNA internal transcribed spacer (ITS) markers

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dc.authoridHaliloglu, Kamil/0000-0002-4014-491X
dc.authoridILHAN, EMRE/0000-0002-8404-7900
dc.authoridKARAHAN, FARUK/0000-0001-7927-1409
dc.authoridTurkoglu, Aras/0000-0003-2611-8034
dc.contributor.authorKarahan, Faruk
dc.contributor.authorIlcim, Ahmet
dc.contributor.authorTurkoglu, Aras
dc.contributor.authorIlhan, Emre
dc.contributor.authorHaliloglu, Kamil
dc.date.accessioned2024-09-18T20:55:39Z
dc.date.available2024-09-18T20:55:39Z
dc.date.issued2022
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractBackground Genus Adonis L. contain approximately 40 annual and perennial species, which are widely distributed in the temperate zones of Asia and Europe, and less frequently in southwestern Asia, northern Africa and the Mediterranean region. The aim of the study was to evaluate the phylogenetic relationship among Adonis taxa collected from Turkiye based on nrDNA Internal transcribed spacer (ITS) markers. Methods Samples of 64 individual genotypes from 21 populations of 10 Adonis taxa were collected from different regions of the country during vegetation period between 2014 and 2018. ITS1, ITS4, P16 and P25 primers within ITS technique was used to genotype the plant materials. Then, genotypic data was used to estimate magnitude and organization of infraspecific variation in different populations of Adonis. Results About 600 bp DNA sequences were obtained from each 64 Adonis genotypes belonging to 21 different populations. The dendrogram obtained from Adonis taxa and out-group sequences had two large main groups. While the out-group species were placed in the first large main group, the sect. Consiligo (perennial) and sect. Adonis (annuals) were placed in different sub-groups of the second large main group. Genetic similarity among Adonis taxa varied between A. microcarpa and A. dentata (98.46%). Principal component analysis indicated that two important components in Adonis taxa genotypes. The expected heterozygosity ranged from 0.0252 (sub-population A) to 0.3460 (sub-population C), with an average of 0.1154. In addition, population differentiation measurements (Fst) ranged from 0.0025 (sub-population C) to 0.9016 (sub-population A) with a relatively high average 0.6601. Conclusions Present analyses revealed that phylogenetic classification (grouping) of Adonis taxa largely depended on morphological structure and present ITS primers were quite efficient in putting forth the genetic diversity of such species. The results of this study suggested that ITS markers could be used in the identification of genetic diversity among the Adonis taxa. The results obtained from molecular data can be used to explore the genetic variation pattern, population structure, and the evolutionary history of genus Adonis in the future.en_US
dc.description.sponsorshipScientific Research Commission of Hatay Mustafa Kemal University [12580]en_US
dc.description.sponsorshipThis study was funded by Scientific Research Commission of Hatay Mustafa Kemal University (Project No: 12580).en_US
dc.identifier.doi10.1007/s11033-022-07607-7
dc.identifier.endpage7826en_US
dc.identifier.issn0301-4851
dc.identifier.issn1573-4978
dc.identifier.issue8en_US
dc.identifier.pmid35678941en_US
dc.identifier.scopus2-s2.0-85131506893en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage7815en_US
dc.identifier.urihttps://doi.org/10.1007/s11033-022-07607-7
dc.identifier.urihttps://hdl.handle.net/20.500.12483/11977
dc.identifier.volume49en_US
dc.identifier.wosWOS:000808398500001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofMolecular Biology Reportsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectRanunculaceaeen_US
dc.subjectPheasant's-eyeen_US
dc.subjectEndemicen_US
dc.subjectMolecular analysesen_US
dc.subjectITSen_US
dc.subjectTurkiyeen_US
dc.titlePhylogenetic relationship among taxa in the genus Adonis L. collected from Turkiye based on nrDNA internal transcribed spacer (ITS) markersen_US
dc.typeArticleen_US

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