EFFECTS OF GENOTYPE AND PICLORAM CONCENTRATIONS ON CALLUS INDUCTION AND PLANT REGENERATION FROM IMMATURE INFLORESCENCE OF SPRING BARLEY CULTIVARS (HORDEUM VULGARE L.)
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Dosyalar
Tarih
2008
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Diagnosis Press Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The effects of different picloram (4-amino-3,5,6-trichloropicolinic acid) concentrations (2.5, 5, 7.5 and 10 mg. l(-1)) in the LS (Linsmaier and Skoog, 8) medium of immature inflorescences taken from four barley (Hordeum vulgare, L.) cultivars were investigated. The result of the study showed that callus induction rate, shoot induction rate, callus weight and plain regeneration front the affected by the genotypes. There was a significant interaction between genotype and immature inflorescence were significantly of picloram concentrations. Depending on the genotypes, mean callus induction rate, mean shoot induction, mean callus weight (mg/petri dishes) and number of regenerates (per inflorescence segment) varied from 0.0-39.1%, 12.5-29.7%, 0.0-150.3 mg and 0.2-1.5 respectively. Mean callus and mean shoot induction rates, mean callus weight and mean regeneration rate were also significantly influenced by the picloram concentrations. The segments cultured on the LS medium containing 7.5 mg l(-1) of picloram gave the highest values of callus induction rate (31.3%), shoot induction rate (40.6%) and plant regeneration (1.2 regenerates per segment). In this study only albino plantlets were obtained.
Açıklama
Anahtar Kelimeler
barley, LS medium, picloram
Kaynak
Biotechnology & Biotechnological Equipment
WoS Q DeÄŸeri
N/A
Scopus Q DeÄŸeri
Cilt
22
Sayı
4
