The genetic diversity of genus Bacillus and the related genera revealed by 16s rRNA gene sequences and ARDRA analyses isolated from geothermal regions of Turkey

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dc.authorscopusid57214957886
dc.authorscopusid37103118100
dc.authorscopusid6603825851
dc.authorscopusid6602504292
dc.contributor.authorCihan, Arzu Coleri
dc.contributor.authorTekin, Nilgun
dc.contributor.authorOzcan, Birgul
dc.contributor.authorCokmus, Cumhur
dc.date.accessioned2024-09-19T15:41:19Z
dc.date.available2024-09-19T15:41:19Z
dc.date.issued2012
dc.departmentHatay Mustafa Kemal Ãœniversitesien_US
dc.description.abstractPreviously isolated 115 endospore-forming bacilli were basically grouped according to their temperature requirements for growth: the thermophiles (74%), the facultative thermophiles (14%) and the mesophiles (12%). These isolates were taken into 16S rRNA gene sequence analyses, and they were clustered among the 7 genera: Anoxybacillus, Aeribacillus, Bacillus, Brevibacillus, Geobacillus, Paenibacillus, and Thermoactinomycetes. Of these bacilli, only the thirty two isolates belonging to genera Bacillus (16), Brevibacillus (13), Paenibacillus (1) and Thermoactinomycetes (2) were selected and presented in this paper. The comparative sequence analyses revealed that the similarity values were ranged as 91.4-100%, 91.8- 99.2%, 92.6- 99.8% and 90.7 - 99.8% between the isolates and the related type strains from these four genera, respectively. Twenty nine of them were found to be related with the validly published type strains. The most abundant species was B. thermoruber with 9 isolates followed by B. pumilus (6), B. lichenformis (3), B. subtilis (3), B. agri (3), B. smithii (2), T. vulgaris (2) and finally P. barengoltzii (1). In addition, isolates of A391a, B51a and D295 were proposed as novel species as their 16S rRNA gene sequences displayed similarities ? 97% to their closely related type strains. The AluI-, HaeIII- and TaqI-ARDRA results were in congruence with the 16S rRNA gene sequence analyses. The ARDRA results allowed us to differentiate these isolates, and their discriminative restriction fragments were able to be determined. Some of their phenotypic characters and their amylase, chitinase and protease production were also studied and biotechnologically valuable enzyme producing isolates were introduced in order to use in further studies.en_US
dc.identifier.doi10.1590/S1517-83822012000100037
dc.identifier.endpage324en_US
dc.identifier.issn1678-4405
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-84861371927en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage309en_US
dc.identifier.urihttps://doi.org/10.1590/S1517-83822012000100037
dc.identifier.urihttps://hdl.handle.net/20.500.12483/14186
dc.identifier.volume43en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.relation.ispartofBrazilian Journal of Microbiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subject16s rRNA geneen_US
dc.subjectARDRAen_US
dc.subjectEndospore-forming bacillien_US
dc.subjectIsolationen_US
dc.subjectTemperature requirementen_US
dc.titleThe genetic diversity of genus Bacillus and the related genera revealed by 16s rRNA gene sequences and ARDRA analyses isolated from geothermal regions of Turkeyen_US
dc.typeArticleen_US

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