Prevalence and molecular characterization of extended-spectrum ?-lactamase (ESBL) and plasmidic AmpC ?-lactamase (pAmpC) producing Escherichia coli in dogs

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dc.contributor.authorAslantas, Ozkan
dc.contributor.authorYilmaz, Ebru Sebnem
dc.date.accessioned2024-09-18T20:15:18Z
dc.date.available2024-09-18T20:15:18Z
dc.date.issued2017
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractThis study aimed to determine the prevalence of fecal carriage of extended spectrum beta-lactamase (ESBL) and/or plasmidic AmpC beta-lactamase (pAmpC) producing Escherichia coli among dogs (n=428) in Turkey. Polymerase chain reaction (PCR) and sequencing were used to characterize genes encoding beta-lactamase and plasmid mediated quinolone resistance (PMQR). Antimicrobial susceptibility testing and PCRs for virulence genes and phylogenetic groups were also performed. Cefotaxime resistant E. coli isolates were detected in 95 (22.2%) of the swab samples. Sequencing analysis results showed occurrence of various beta-lactamase genes: bla(CTX-M-15) (62), bla(TEM-1b) (42), bla(CMY-2) (22), bla(CTX-M-3) (16), bla(CTX-M-1) (15), bla(OXA-1) (9) and bla(SHV-12) (3) alone or in combination. The most frequently encountered phylogenetic group was group A(1) (35.8%), followed by group D-2 (22.1%), B1 (15.8%), D-1 (9.5%), A(0) (7.4%), B2(2) (5.3%) and B2(3) (4.2%), respectively. PMQR genes, aac(6')-Ib-cr, qnrS1 and qnrB10 were detected in 25.3, 10.5 and 1.1% of the isolates, respectively. While all isolates were susceptible to imipenem and amikacin, resistance rates to non-beta-lactam antibiotics ranged from 20.0% for tobramycin to 56.8% for tetracycline. The virulence genes were only detected in 34 (36.2%) of the isolates and this isolates carried single or various combination of virulence genes of iucD, papC, papE, f17a-A and eaeA. Four isolates were identified as human virulent pandemic CTX-M-15 producing E. coli clone O25b:ST131/B2. To the best of our knowledge, this is the first study to show fecal carriage of ESBL/pAmpC type beta-lactamase producing E. coli isolates among dogs in Turkey.en_US
dc.description.sponsorshipScientific and Technical Research Council of Turkey (TUBITAK) [TOVAG 113 O 843]en_US
dc.description.sponsorshipThe study was funded by the Scientific and Technical Research Council of Turkey (TUBITAK, Project Number: TOVAG 113 O 843).en_US
dc.identifier.doi10.1292/jvms.16-0432
dc.identifier.endpage1030en_US
dc.identifier.issn0916-7250
dc.identifier.issn1347-7439
dc.identifier.issue6en_US
dc.identifier.pmid28450661en_US
dc.identifier.scopus2-s2.0-85020532764en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1024en_US
dc.identifier.urihttps://doi.org/10.1292/jvms.16-0432
dc.identifier.urihttps://hdl.handle.net/20.500.12483/9556
dc.identifier.volume79en_US
dc.identifier.wosWOS:000406226200012en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherJapan Soc Vet Scien_US
dc.relation.ispartofJournal of Veterinary Medical Scienceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectdogen_US
dc.subjectEscherichia colien_US
dc.subjectESBLen_US
dc.subjectmolecular characterizationen_US
dc.subjectpAmpCen_US
dc.titlePrevalence and molecular characterization of extended-spectrum ?-lactamase (ESBL) and plasmidic AmpC ?-lactamase (pAmpC) producing Escherichia coli in dogsen_US
dc.typeArticleen_US

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