Comparision of pure nicotine and smokeless tobacco extract induced formation of 8-OH-dG

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dc.contributor.authorYildiz, D
dc.date.accessioned2024-09-18T20:52:45Z
dc.date.available2024-09-18T20:52:45Z
dc.date.issued2004
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractNicotine and smokeless tobacco extract have been shown to induce oxidative stress in different experimental systems. However, the effect of nicotine and smokeless tobacco extract containing equal amounts of nicotine on 8-OH-dG formation has not been investigated. 8-OH-dG is a DNA adduct formed by free radical attack and is elevated in tumor cells. The objective of the present study was to evaluate the formation of 8-OH-dG following exposure to different concentrations of nicotine and smokeless tobacco extract containing equal amounts of nicotine. Exposure of Chinese Hamster Ovary cells to 5 and 10 mM of nicotine resulted in generation of 8-OH-dG. The observed 8-OH-dG levels at these concantrations of nicotine were 5.1 +/- 1.7 and 9.4 +/- 2.3 8-OH-dG/dG x 100.000, respectively. Smokeless tobacco extract containing 1 and 5 mM nicotine also induced generation of 8-OH-dG. The measured 8-OH- dG levels at these concentrations were 4.7 +/- 0.6 and 20.6 +/- 2.2 8-OH- dG/dG x 100.000 respectively. Exposure of cells to smokeless tobacco extract containing 10 mM nicotine resulted in cell death. Coaddition of superoxide dismutase and catalase along with nicotine reversed the 8-OH- dG generation. However, superoxide dismutase and catalase did not inhibit the 8-OH- dG generation in smokeless tobacco extract treated cells.en_US
dc.identifier.doi10.1080/15376520490434700
dc.identifier.endpage256en_US
dc.identifier.issn1537-6524
dc.identifier.issue4en_US
dc.identifier.pmid20021139en_US
dc.identifier.scopus2-s2.0-3543001089en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage253en_US
dc.identifier.urihttps://doi.org/10.1080/15376520490434700
dc.identifier.urihttps://hdl.handle.net/20.500.12483/11367
dc.identifier.volume14en_US
dc.identifier.wosWOS:000222551100008en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofToxicology Mechanisms and Methodsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectnicotineen_US
dc.subjectoxidative DNA damageen_US
dc.subjectsmokeless tobacco extracten_US
dc.titleComparision of pure nicotine and smokeless tobacco extract induced formation of 8-OH-dGen_US
dc.typeArticleen_US

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