Biofilm formation, antibiotic resistance and genotyping of Shiga toxin-producing Escherichia coli isolated from retail chicken meats

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dc.authoridERTAS ONMAZ, Nurhan/0000-0002-4679-6548
dc.authoridgonulalan, zafer/0000-0002-3935-6296
dc.authoridDisli, Huseyin Burak/0000-0001-8255-3802
dc.authoridBarel, Mukaddes/0000-0002-1170-8632
dc.authoridHIZLISOY, Harun/0000-0003-3391-0185
dc.authoridDishan, Adalet/0000-0001-8097-1648
dc.authoridGungor, Candan/0000-0002-4321-2770
dc.contributor.authorDishan, A.
dc.contributor.authorHizlisoy, H.
dc.contributor.authorBarel, M.
dc.contributor.authorDisli, H. B.
dc.contributor.authorGungor, C.
dc.contributor.authorOnmaz, N. Ertas
dc.contributor.authorGonulalan, Z.
dc.date.accessioned2024-09-18T19:54:28Z
dc.date.available2024-09-18T19:54:28Z
dc.date.issued2023
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstract1. The Shiga toxin-producing Escherichia coli (STEC) is a hazardous zoonotic agent for chicken meat consumers. This study determined the serogroups and evaluated the virulence genes, antibiotic resistance, biofilm-forming profiles and genetic relationships of STEC isolates in chicken meat. 2. A total of 100 samples belonging to dressed-whole chicken and different parts of the chicken (wing, breast, thigh, drumstick) were collected between September and November 2019 from different retail markets in Kayseri, Turkiye. 3. Phenotypic (identification, disc diffusion test, Congo red agar and microtitre plate tests) and molecular tests (identification, serogrouping, virulence factors, biofilm, antibiotic susceptibility, 16S rRNA sequencing and enterobacterial repetitive intergenic consensus-PCR for typing of the isolates) were carried out. 4. E. coli was isolated from 35% of the samples and 35% of the samples harboured at least one STEC. Among 35 STEC isolates, 3 (8.5%), 6 (17.1%), 2 (5.7%) and 3 (8.5%) were found to be positive for fliCH2, fliCH8, fliCH11, fliCH19 genes, respectively. Out of 35 STEC positive isolates, 4 (11.4%) were identified as E. coli O157, from which 2 (5.7%) were E. coli O157:H7. E. coli O157 was detected in two (10%), one (5%), one (5%) of the thigh, drumstick and whole chicken samples, respectively. 5. Biofilm-forming ability was reported in 33 (94.2%) of 35 E. coli isolates, whilst the biofilm-associated genes detected among 35 STEC isolates included csgA (88.5%), fimH (88.5%), bcsA (85.7%), agn43 (14.2%) and papC (8.5%). The STEC strains showed resistance against ampicillin (88.5%) and erythromycin (88.5%), followed by tetracycline (74.2%) and gentamicin (25.7%). However, the distribution of isolates harbouring bla(CMY), ere(A), tet(A) and aac(3)-IV antibiotic resistance genes was found to be 17.1%, 11.4%, 85.7% and 5.7%, respectively. 6. ERIC-PCR showed that E. coli strains obtained from different parts and whole of chicken samples had genetic diversities. ERIC-PCR patterns grouped strains of 35 STEC into eight clusters designated A-H, with 73% similarity. Proper hygiene measures and staff training are essential for public health during poultry processing and in retail stores to control STEC.en_US
dc.identifier.doi10.1080/00071668.2022.2116697
dc.identifier.endpage73en_US
dc.identifier.issn0007-1668
dc.identifier.issn1466-1799
dc.identifier.issue1en_US
dc.identifier.pmid36102939en_US
dc.identifier.scopus2-s2.0-85141189846en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage63en_US
dc.identifier.urihttps://doi.org/10.1080/00071668.2022.2116697
dc.identifier.urihttps://hdl.handle.net/20.500.12483/7758
dc.identifier.volume64en_US
dc.identifier.wosWOS:000870153000001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofBritish Poultry Scienceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAntibiotic resistanceen_US
dc.subjectbiofilm-associated genesen_US
dc.subjectERIC-PCRen_US
dc.subjectSTECen_US
dc.subjectvirulenceen_US
dc.titleBiofilm formation, antibiotic resistance and genotyping of Shiga toxin-producing Escherichia coli isolated from retail chicken meatsen_US
dc.typeArticleen_US

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