Fertility comparison of frozen bull semen stored in cryogenic deep freezer (-152 °C) and LN2 container

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dc.authoridOLGAC, KEMAL TUNA/0000-0001-9216-7059
dc.authoridKaya, Ufuk/0000-0002-4805-0993
dc.authoridTIRPAN, Mehmet Borga/0000-0001-8782-1108
dc.authoridKorkmaz, Firat/0000-0002-9600-908X
dc.contributor.authorTirpan, Mehmet Borga
dc.contributor.authorOlgac, Kemal Tuna
dc.contributor.authorKorkmaz, Firat
dc.contributor.authorSonat, Ali
dc.contributor.authorKaya, Ufuk
dc.contributor.authorAkcay, Ergun
dc.date.accessioned2024-09-18T20:11:43Z
dc.date.available2024-09-18T20:11:43Z
dc.date.issued2022
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractThe present study aimed to use cryogenic deep freezers that could be a feasible alternative for cryopreserved semen storage. A total of 284 straws from three Simmental bulls and 272 Simmental cows were used. The experimental group consisted of 151 semen straws that were stored at -152 & DEG;C for a week. Moreover, the control group consisted of 133 semen straws that were stored at -196 & DEG;C. Firstly, two samples per bull (n = 6) were examined in terms of sperm kinetic parameters by CASA. Furthermore, plasma membrane, acrosome integrity (PMAI) and high mitochondrial membrane potential (HMMP) were analyzed by flow cytometry. Then, artificial inseminations were performed on Simmental cows with 272 straws belonging to two groups. Then, 56th-day Non-return Rate (NRR56) was determined. All spermatological data were subjected to a linear mixed model. Chi-Square test was performed to NRR56 between storage temperature groups. Also, logistic regression analysis was used to examine the effect of bull, storage temperature and age of cows on pregnancy status. While age of cows was included in the final logistic regression model, effect of bull x storage temperature was not included because it was found as non-significant. The post-thaw PMOT and STR of cryopreserved bull semen, which was stored at -152 & DEG;C, had lower and statistically significant values (p < 0.05). However, frozen bull semen, which were stored at -152 & DEG;C, kept its fertility ability as which stored at -196 & DEG;C. Besides, NRR56 of semen stored at -152 & DEG;C and -196 & DEG;C were detected as 57.24% (83/145) and 55.91% (71/127), respectively (p > 0.05). Nevertheless, these results should be supplemented with more pre-freezing and post-thaw sperm quality analyses and more fertility data for increasing the accuracy of the method. (C)& nbsp;2022 Elsevier Inc. All rights reserved.en_US
dc.identifier.doi10.1016/j.theriogenology.2022.03.017
dc.identifier.endpage5en_US
dc.identifier.issn0093-691X
dc.identifier.issn1879-3231
dc.identifier.pmid35344831en_US
dc.identifier.scopus2-s2.0-85126917401en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1en_US
dc.identifier.urihttps://doi.org/10.1016/j.theriogenology.2022.03.017
dc.identifier.urihttps://hdl.handle.net/20.500.12483/9038
dc.identifier.volume185en_US
dc.identifier.wosWOS:000791190700001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Incen_US
dc.relation.ispartofTheriogenologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectComputer-assisted sperm analyzeren_US
dc.subjectCryogenic freezeren_US
dc.subjectFlow cytometryen_US
dc.subjectFrozen bull semenen_US
dc.subjectNon-return rate 56en_US
dc.subjectSemen storageen_US
dc.titleFertility comparison of frozen bull semen stored in cryogenic deep freezer (-152 °C) and LN2 containeren_US
dc.typeArticleen_US

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