Comparison of inflammatory cytokine release from nasal epithelial cells of non-atopic non-rhinitic, allergic rhinitic and polyp subjects and effects of diesel exhaust particles in vitro

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dc.authoridBayram, Hasan/0000-0002-5236-766X
dc.authoridBayraktar, Recep/0000-0002-8227-2055
dc.contributor.authorOzturk, A. B.
dc.contributor.authorBayraktar, R.
dc.contributor.authorGogebakan, B.
dc.contributor.authorMumbuc, S.
dc.contributor.authorBayram, H.
dc.date.accessioned2024-09-18T19:52:36Z
dc.date.available2024-09-18T19:52:36Z
dc.date.issued2017
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractBackground: Although studies have reported an association between air pollutants and increased allergic airway diseases, such as allergic rhinitis and nasal polyposis, the underlying mechanisms are not fully understood. A limited number of studies have suggested that diesel exhaust particles (DEP) play a role in atopy and the pathogenesis of allergic upper airway diseases. The aim of this study was to investigate the effect of DEP on inflammatory cytokine release, and mRNA expression of transcription factors such as JNK and NF-beta in primary nasal epithelial cells (NECs), in vitro. Methods: NECs from non-atopic, non-rhinitic subjects (controls) and patients with allergic rhinitis and nasal polyps were cultured and incubated with 0-100 mu g/nril DEP for 24 h. ELISA and RT-PCR were used to assess the release of IL-8, GM-CSF, and RANTES, and mRNA expression for JNK and NF-kappa B, respectively. Results: Compared to control cells, NECs from subjects with atopic polyps released significantly greater amounts of IL-8 (median = 887 vs. 176.6 pg/mu g cellular protein; p < 0.0001) and RANTES (median = 0.191 vs. 0.02 pg/mu g cellular protein; p < 0.001). While 50 mu g/ml DEP induced release of RANTES in NECs from patients with allergic rhinitis, 100 mu g/ml DEP decreased IL-8 levels in NECs from both control and allergic rhinitic subjects. DEP did not affect mRNA expression for JNK and NE-kappa B from NECs of subjects with polyps. Conclusions: NECs from subjects with various pathologies may respond differently to DEP. (C) 2017 SEICAR Published by Elsevier Espana, S.L.U. All rights reserved.en_US
dc.description.sponsorshipUniversity of Gaziantep [TF.11.29]; Scientific and Technological Research Council of Turkey (TUBITAK) [111S251]en_US
dc.description.sponsorshipThese studies were funded by research grants from The University of Gaziantep (Grant no: TF.11.29), and The Scientific and Technological Research Council of Turkey (TUBITAK) (Grant no: 111S251).en_US
dc.identifier.doi10.1016/j.aller.2016.10.015
dc.identifier.endpage481en_US
dc.identifier.issn0301-0546
dc.identifier.issn1578-1267
dc.identifier.issue5en_US
dc.identifier.pmid28259510en_US
dc.identifier.scopus2-s2.0-85014129284en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage473en_US
dc.identifier.urihttps://doi.org/10.1016/j.aller.2016.10.015
dc.identifier.urihttps://hdl.handle.net/20.500.12483/7547
dc.identifier.volume45en_US
dc.identifier.wosWOS:000411656400010en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Doyma Slen_US
dc.relation.ispartofAllergologia Et Immunopathologiaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAllergic rhinitisen_US
dc.subjectAir pollutantsen_US
dc.subjectDiesel exhausten_US
dc.subjectInflammationen_US
dc.subjectNasal polypen_US
dc.titleComparison of inflammatory cytokine release from nasal epithelial cells of non-atopic non-rhinitic, allergic rhinitic and polyp subjects and effects of diesel exhaust particles in vitroen_US
dc.typeArticleen_US

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