Comparison of cryosurvival and spermatogenesis efficiency of cryopreserved neonatal mouse testicular tissue between three vitrification protocols and controlled-rate freezing

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dc.contributor.authorYildiz, Cengiz
dc.contributor.authorMullen, Brendan
dc.contributor.authorJarvi, Keith
dc.contributor.authorMcKerlie, Colin
dc.contributor.authorLo, Kirk C.
dc.date.accessioned2024-09-18T20:32:58Z
dc.date.available2024-09-18T20:32:58Z
dc.date.issued2018
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractGrafting of cryopreserved testicular tissue is a promising tool for fertility and testicular function preservation in endangered species, mutant animals, or cancer patients for future use. In this study, we aimed to improve the whole neonatal mouse testicular tissue cryopreservation protocols by comparing cryosurvival, spermatogenesis, and androgen production of grafted testicular tissue after cryopreservation with three different vitrification protocols and an automated computed controlled-rate freezing. Whole neonatal mouse testes were vitrified with various vitrification solutions (V1) 40% EG + 18% Ficoll + 0.35 M Sucrose, (V2) DAP 213 (2 M DMSO + 1 M Acetamid + 3 M PG), or (V3) 15% EG + 15% PG + 0.5 M Sucrose (total solute concentration V1:74.34%, V2:44.0%, and V3:49.22% wt/vol). Alternatively, neonatal testicular tissue was also frozen in 0.7 M DMSO + 5% fetal bovine serum using controlled-rate freezing and compared to fresh grafted testicular tissue, sham grafted controls, and the vitrification protocol groups. Fresh (n = 4) and frozen-thawed (n = 4) testes tissues were grafted onto the flank of castrated male NCr Nude recipient mouse. The grafts were harvested after three months. Fresh or frozen-thawed grafts with controlled-rate freezing had the highest rate of tissue survival compared to other vitrified protocols after harvesting (p < 0.05). Both controlled-rate freezing and V1 protocol groups displayed the most advanced stages of spermatogenesis with elongated spermatids and spermatozoa in 17.6 +/- 1.3% and 16.3 +/- 1.9% of seminiferous tubules based on histopathological evaluation, respectively. Hosts of the testicular graft from controlled-rate freezing had higher levels of serum testosterone compared to all other vitrified-thawed graft groups (p < 0.05). This study shows that completed spermatogenesis from whole neonatal mouse testes were obtained when frozen with controlled-rate freezing and V1 vitrification solution and that testicular cryopreservation efficacy vary with the protocol and vitrification technique.en_US
dc.description.sponsorshipCanadian Urological Association Scholarship Funden_US
dc.description.sponsorshipThis project is funded by the Canadian Urological Association Scholarship Fund.en_US
dc.identifier.doi10.1016/j.cryobiol.2018.09.001
dc.identifier.endpage9en_US
dc.identifier.issn0011-2240
dc.identifier.issn1090-2392
dc.identifier.pmid30195700en_US
dc.identifier.scopus2-s2.0-85053109382en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage4en_US
dc.identifier.urihttps://doi.org/10.1016/j.cryobiol.2018.09.001
dc.identifier.urihttps://hdl.handle.net/20.500.12483/11243
dc.identifier.volume84en_US
dc.identifier.wosWOS:000446416200002en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAcademic Press Inc Elsevier Scienceen_US
dc.relation.ispartofCryobiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMouse testesen_US
dc.subjectSpermatogenesisen_US
dc.subjectSpermatozoaen_US
dc.subjectGraftingen_US
dc.subjectCryopreservationen_US
dc.subjectVitrificationen_US
dc.subjectTestosteroneen_US
dc.titleComparison of cryosurvival and spermatogenesis efficiency of cryopreserved neonatal mouse testicular tissue between three vitrification protocols and controlled-rate freezingen_US
dc.typeArticleen_US

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