Phenol biodegradation by halophilic archaea

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dc.authoridAcikgoz, Eda/0000-0002-6772-3081
dc.contributor.authorAcikgoz, Eda
dc.contributor.authorOzcan, Birgul
dc.date.accessioned2024-09-18T20:26:33Z
dc.date.available2024-09-18T20:26:33Z
dc.date.issued2016
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractPhenol is a toxic aromatic compound produced as a by-product of industrial activities. Biological treatment of highly saline wastewaters containing phenol can be performed through halophilic microorganisms. In this study, the ability of halophilic archaeal isolates to degrade phenol was investigated. Among 103 tested isolates, the strain designated A235 was identified as having the highest phenol degradation capacity on solid and liquid media containing 20% (w/v) NaCl and phenol as the sole carbon and energy source. The strain was adapted sequentially to increasing phenol concentrations. The removal of phenol via cross-toluene adaptation was increased by 14% in the medium. The growth kinetics of strain A235 during growth on phenol was found to fit the Monod model. The values of mu max and Ks were calculated to be 0.015 h(-1) and 71.4 g l(-1), respectively. For an initial phenol concentration of 100 ppm, the biodegradation by A235 was found to be optimal at pH 7.5, 37 degrees C and 200 rpm when the culture contained 20% (w/v) NaCl, 0.025% yeast extract and the inoculum size was set at 10%. A preliminary enzyme screening indicated that the degradation of phenol was achieved through a meta-cleavage pathway involving a catechol 2,3-dioxygenase. Catechol 2,3-dioxygenase displayed its highest catalytic activity at 42 degrees C, 2 M KCl, and pH 8. To the best of our knowledge, this is the first report showing the ability an extremely halophilic archaeon to metabolize phenol at higher salt concentrations. (C) 2015 Elsevier Ltd. All rights reserved.en_US
dc.description.sponsorshipResearch Project Units of Mustafa Kemal University [1105Y0106]en_US
dc.description.sponsorshipThis study was supported by the Research Project Units of Mustafa Kemal University (project number 1105Y0106).en_US
dc.identifier.doi10.1016/j.ibiod.2015.11.016
dc.identifier.endpage146en_US
dc.identifier.issn0964-8305
dc.identifier.issn1879-0208
dc.identifier.scopus2-s2.0-84948971278en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage140en_US
dc.identifier.urihttps://doi.org/10.1016/j.ibiod.2015.11.016
dc.identifier.urihttps://hdl.handle.net/20.500.12483/10390
dc.identifier.volume107en_US
dc.identifier.wosWOS:000369206700019en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevier Sci Ltden_US
dc.relation.ispartofInternational Biodeterioration & Biodegradationen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectHalophilic archaeaen_US
dc.subjectPhenol biodegradationen_US
dc.subjectAdaptationen_US
dc.subjectCatechol 2,3-dioxygenaseen_US
dc.titlePhenol biodegradation by halophilic archaeaen_US
dc.typeArticleen_US

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