Immunohistochemical analysis of thyroid follicular neoplasms and BRAF mutation correlation

Basit Öğe Kaydı
dc.contributor.authorAtik, E.
dc.contributor.authorGuray, M.
dc.contributor.authorGunesacar, R.
dc.contributor.authorOzgur, T.
dc.contributor.authorCanda, T.
dc.date.accessioned2024-09-18T20:06:10Z
dc.date.available2024-09-18T20:06:10Z
dc.date.issued2014
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractBackground: The accurate diagnosis of benign and malign thyroid tumors is very important for the clinical management of patients. The distinction of thyroid papillary carcinoma follicular variant and follicular adenoma can be difficult. Aim: To investigate the alternative methods like immunohistochemistry and exon 15 in the BRAF gene 1799 T/A mutation analyses for distinguishing thyroid tumors. Materials and Methods: We applied immunohistochemical markers; CK19, HMWCK, Galectin-3, HBME-1 and Fibronectin and mutant allele-specific PCR amplification technique was used to determine 1799 T/A mutation within the BRAF gene. Formalin-fixed parafin embedded tissues from 45 surgically total resected thyroids, included 26 thyroid papillary carcinoma follicular variant (FV-TPC), 8 Follicular Adenoma (FA), 6 Minimal invasive follicular carcinoma (MIFC) and 5 Follicular Carcinoma (FC). Statistical Analyses Used: Pearson Chi-Square and Kruskal Wallis tests were performed. Results: There was a positive correlation between FV-TPC and HMWCK, CK 19, HBME1, Galectin 3, fibronectin (P < 0.05), but there was no correlation with FV-TPC and BRAF gene mutation (P > 0.05). HBME-1 and CK 19 stained strong and diffuse positive in FV-TPCs but weak and focal in FAs. Conclusion: Our study suggests that morphologic features combined with immunohistochemical panel of HMWCK, CK19, HBME-1, Galectin-3 and fibronectin can help to distinguish benign and malign thyroid neoplasms and FV-TPC from follicular adenomas. BRAF gene 1799 T/A mutation has been non-specific but its detection can be a useful tool combined with immunohistochemistry for diagnosing FV-TPC.en_US
dc.identifier.doi10.4103/0019-509X.134648
dc.identifier.endpage68en_US
dc.identifier.issn0019-509X
dc.identifier.issn1998-4774
dc.identifier.issue1en_US
dc.identifier.pmid24947099en_US
dc.identifier.scopus2-s2.0-84903291688en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage63en_US
dc.identifier.urihttps://doi.org/10.4103/0019-509X.134648
dc.identifier.urihttps://hdl.handle.net/20.500.12483/8343
dc.identifier.volume51en_US
dc.identifier.wosWOS:000339909800014en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherIndian Cancer Socen_US
dc.relation.ispartofIndian Journal of Canceren_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBRAFen_US
dc.subjectImmunohistochemistryen_US
dc.subjectpapillary carcinoma follicular varianten_US
dc.subjectthyroiden_US
dc.titleImmunohistochemical analysis of thyroid follicular neoplasms and BRAF mutation correlationen_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
Tam Metin / Full text
Boyut:
3.8 MB
Biçim:
Adobe Portable Document Format
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu