Leishmaniasis in Turkey: Determination of Leishmania Species by Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS)

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dc.authoridGunduz, Cumhur/0000-0002-6593-3237
dc.authoridcavus, ibrahim/0000-0002-3860-0146
dc.authoridKurt, Ozgur/0000-0001-5575-588X
dc.contributor.authorCulha, Gulnaz
dc.contributor.authorAkyar, Isin
dc.contributor.authorZeyrek, Fadile Yildiz
dc.contributor.authorKurt, Ozgur
dc.contributor.authorGunduz, Cumhur
dc.contributor.authorToz, Seray Ozensoy
dc.contributor.authorOstan, Ipek
dc.date.accessioned2024-09-18T20:13:41Z
dc.date.available2024-09-18T20:13:41Z
dc.date.issued2014
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractBackground: Cutaneous leishmaniasis (CL) is endemic in Southeastern Anatolia, mainly in Sanliurfa and Hatay provinces, and the causative agents are mostly Leishmania tropica and less frequently L. infantum. Here, we report the first MALDI-TOF analyses of Leishmania promastigotes obtained from the cultures of two CL cases from Osmaniye and Hatay provinces who were initially diagnosed by microscopy, culture and identified as L. infantum with Real-Time PCR (RT-PCR). Methods: Samples obtained from the skin lesions of patients were initially stained with Giemsa and cultivated in NNN medium. Examination of the smears and cultures revealed Leishmania amastigotes and promastigotes, respectively. The promastigotes (MHOM/TR/2012/CBU15 and MHOM/TR/2012/MK05) obtained from the cultures of both patients were used for RT-PCR targeting the ITS-1 region in the SSU of rRNA. The reference strains of four Leishmania species (L. infantum, L. donovani, L. tropica and L. major) were initially assessed with MALDI-TOF and their data were added to MALDI-TOF Biotyper Library. Results: Both RT-PCR and MALDI-TOF analyses indicated that the causative agent in both patient samples was L. infantum. Conclusion: Despite disadvantages such as requirement of culture fluid with nothing but promastigotes and high cost, MALDI-TOF analysis may be a fast, sensitive and specific diagnostic tool in especially large-scale research studies, where the cost declines, relatively.en_US
dc.description.sponsorshipTUBITAK (The Scientific and Technological Research Council of Turkey) [111S179]en_US
dc.description.sponsorshipThis study is supported by TUBITAK [(The Scientific and Technological Research Council of Turkey) (Project No: 111S179)]. MALDI-TOF analyses in the present study were conducted in Acibadem Labmed Clinical Laboratories. The authors wish to thank to Professor Ibrahim Unsal, the director of Acibadem Labmed Clinical Laboratories, for his support to the present study. We also wish to thank laboratory technician Simge Can for her contribution during MALDI-TOF analyses. The authors declare that there is no conflict of interests.en_US
dc.identifier.endpage248en_US
dc.identifier.issn1735-7020
dc.identifier.issn2008-238X
dc.identifier.issue2en_US
dc.identifier.pmid25848391en_US
dc.identifier.startpage239en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12483/9320
dc.identifier.volume9en_US
dc.identifier.wosWOS:000337343400013en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherIranian Scientific Society Medical Entomologyen_US
dc.relation.ispartofIranian Journal of Parasitologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCutaneous leishmaniasisen_US
dc.subjectLeishmania infantumen_US
dc.subjectTurkeyen_US
dc.subjectReal-Time PCRen_US
dc.subjectMALDI-TOFen_US
dc.titleLeishmaniasis in Turkey: Determination of Leishmania Species by Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS)en_US
dc.typeArticleen_US

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